Please use this identifier to cite or link to this item: doi:10.22028/D291-39920
Title: Red blood cell lingering modulates hematocrit distribution in the microcirculation
Author(s): Rashidi, Yazdan
Simionato, Greta
Zhou, Qi
John, Thomas
Kihm, Alexander
Bendaoud, Mohammed
Krüger, Timm
Bernabeu, Miguel O.
Kaestner, Lars
Laschke, Matthias W.
Menger, Michael D.
Wagner, Christian
Darras, Alexis
Language: English
Title: Biophysical Journal
Volume: 122
Issue: 8
Pages: 1526-1537
Publisher/Platform: Elsevier
Year of Publication: 2023
DDC notations: 500 Science
610 Medicine and health
Publikation type: Journal Article
Abstract: The distribution of red blood cells (RBCs) in the microcirculation determines the oxygen delivery and solute transport to tissues. This process relies on the partitioning of RBCs at successive bifurcations throughout the microvascular network, and it has been known since the last century that RBCs partition disproportionately to the fractional blood flow rate, therefore leading to heterogeneity of the hematocrit (i.e., volume fraction of RBCs in blood) in microvessels. Usually, downstream of a microvascular bifurcation, the vessel branch with a higher fraction of blood flow receives an even higher fraction of RBC flux. However, both temporal and time-average deviations from this phase-separation law have been observed in recent studies. Here, we quantify how the microscopic behavior of RBC lingering (i.e., RBCs temporarily residing near the bifurcation apex with diminished velocity) influences their partitioning, through combined in vivo experiments and in silico simulations. We developed an approach to quantify the cell lingering at highly confined capillary-level bifurcations and demonstrate that it correlates with deviations of the phase-separation process from established empirical predictions by Pries et al. Furthermore, we shed light on how the bifurcation geometry and cell membrane rigidity can affect the lingering behavior of RBCs; e.g., rigid cells tend to linger less than softer ones. Taken together, RBC lingering is an important mechanism that should be considered when studying how abnormal RBC rigidity in diseases such as malaria and sickle-cell disease could hinder the microcirculatory blood flow or how the vascular networks are altered under pathological conditions (e.g., thrombosis, tumors, aneurysm).
DOI of the first publication: 10.1016/j.bpj.2023.03.020
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-399209
ISSN: 0006-3495
Date of registration: 7-Jun-2023
Description of the related object: Supporting material
Related object:
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Anatomie und Zellbiologie
M - Chirurgie
NT - Physik
Professorship: M - Prof. Dr. Michael D. Menger
M - Keiner Professur zugeordnet
NT - Prof. Dr. Christian Wagner
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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