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Titel: Mechanistic aspects of functional layer formation in hybrid one-step designed GOx/Nafion/Pd-NPs nanobiosensors
VerfasserIn: Butyrskaya, E. V.
Korkmaz, N.
Zolotukhina, E. V.
Krasiukova, V.
Silina, Y. E.
Sprache: Englisch
Titel: The Analyst
Bandnummer: 146
Heft: 7
Seiten: 2172-2185
Verlag/Plattform: Royal Society of Chemistry
Erscheinungsjahr: 2021
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Amperometric nanobiosensors are crucial time and cost effective analytical tools for the detection of a wide range of bioanalytes, viz. glucose present in complex environments at very low concentrations. Although the excellent analytical performance of nanobiosensors is undoubted, their exact molecular structure often remains unclear. Here, by combining advanced nanoanalytical approaches with theoretical modeling, we conducted a comprehensive study towards the investigation of the molecular structure of a hybrid GOx/Nafion/Pd-NPs layer deposited by electroplating from the multicomponent electrolyte solution on the surface of screen printed electrodes modified with graphene oxide. Specifically, we revealed that Pd2+ cations were adsorbed on GOx amino acid residues, forming the GOx·nPd2+ enzymatic complex. The highest adsorption energy of Pd2+ cations on GOx was found during their interaction with the side chains of basic amino acids and methionine. In addition, we showed and fully validated the endstructure of the one-step designed GOx/Nafion/Pd-NPs nanobiosensor as a structural model mainly composed of GOx and water molecules incorporated into the metal–polymer scaffold. Our approach will thus serve as a guideline for the study of molecular interactions occurring in complex systems and will contribute to the design of the next generation of hybrid nanobiosensors. The proposed mechanism, driving the self-assembly of the hybrid layer, will allow us to construct modular enzymatic nanoanalytical devices with tailored sequences in the future.
DOI der Erstveröffentlichung: 10.1039/D0AN02429E
URL der Erstveröffentlichung: https://doi.org/10.1039/D0AN02429E
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-397406
hdl:20.500.11880/35807
http://dx.doi.org/10.22028/D291-39740
ISSN: 1364-5528
0003-2654
Datum des Eintrags: 11-Mai-2023
Bezeichnung des in Beziehung stehenden Objekts: Electronic supplementary information
In Beziehung stehendes Objekt: https://www.rsc.org/suppdata/d0/an/d0an02429e/d0an02429e1.pdf
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Bruce Morgan
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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