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Titel: Ca2+-activated Cl- currents in the murine vomeronasal organ enhance neuronal spiking but are dispensable for male-male aggression
VerfasserIn: Münch, Jonas
Billig, Gwendolyn
Hübner, Christian A.
Leinders-Zufall, Trese
Zufall, Frank
Jentsch, Thomas J.
Sprache: Englisch
Titel: The Journal of Biological Chemistry
Bandnummer: 293
Heft: 26
Seiten: 10392-10403
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2018
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Ca2-activated Cl currents have been observed in many physiological processes, including sensory transduction in mammalian olfaction. The olfactory vomeronasal (or Jacobson’s) organ (VNO) detects molecular cues originating from animals of the same species or from predators. It then triggers innate behaviors such as aggression, mating, or flight. In the VNO, Ca2-activated Cl channels (CaCCs) are thought to amplify the initial pheromone-evoked receptor potential by mediating a depolarizing Cl efflux. Here, we confirmed the co-localization of the Ca2-activated Cl channels anoctamin 1 (Ano1, also called TMEM16A) and Ano2 (TMEM16B) in microvilli of apically and basally located vomeronasal sensory neurons (VSNs) and their absence in supporting cells of the VNO. Both channels were expressed as functional isoforms capable of giving rise to Ca2-activated Cl currents. Although these currents persisted in the VNOs of mice lacking Ano2, they were undetectable in olfactory neuron-specific Ano1 knockout mice irrespective of the presence of Ano2. The loss of Ca2- activated Cl currents resulted in diminished spontaneous and drastically reduced pheromone-evoked spiking of VSNs. Although this indicated an important role of anoctamin channels in VNO signal amplification, the lack of this amplification did not alter VNO-dependent male–male territorial aggression in olfactory Ano1/Ano2 double knockout mice. We conclude that Ano1 mediates the bulk of Ca2-activated Cl currents in the VNO and that Ano2 plays only a minor role. Furthermore, vomeronasal signal amplification by CaCCs appears to be dispensable for the detection of male-specific pheromones and for near-normal aggressive behavior in mice.
DOI der Erstveröffentlichung: 10.1074/jbc.RA118.003153
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/pii/S0021925820338722
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-397278
hdl:20.500.11880/35799
http://dx.doi.org/10.22028/D291-39727
ISSN: 0021-9258
Datum des Eintrags: 10-Mai-2023
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://ars.els-cdn.com/content/image/1-s2.0-S0021925820338722-mmc1.pdf
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Physiologie
Professur: M - Prof. Dr. Trese Leinders-Zufall
M - Prof. Dr. Frank Zufall
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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