Please use this identifier to cite or link to this item: doi:10.22028/D291-39630
Title: Endotoxin Tolerance Acquisition and Altered Hepatic Fatty Acid Profile in Aged Mice
Author(s): Wiesenthal, Amanda A.
Legroux, Thierry M.
Richter, Chris
Junker, Björn H.
Hecksteden, Anne
Kessler, Sonja M.
Hoppstädter, Jessica
Kiemer, Alexandra K.
Language: English
Title: Biology
Volume: 12
Issue: 4
Publisher/Platform: MDPI
Year of Publication: 2023
Free key words: LPS tolerance
hepatic fatty acids
immune response
fatty acid elongation
liver and lung tissue
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: (1) Background: Aging is linked to an altered immune response and metabolism. Inflammatory conditions, such as sepsis, COVID-19, and steatohepatitis are more prevalent in the elderly and steatosis is linked both to severe COVID-19 and sepsis. We hypothesized that aging is linked to a loss of endotoxin tolerance, which normally protects the host from excessive inflammation, and that this is accompanied by elevated levels of hepatic lipids. (2) Methods: An in vivo lipopolysaccharide (LPS) tolerance model in young and old mice was used and the cytokine serum levels were measured by ELISA. Cytokine and toll-like receptor gene expression was determined by qPCR in the lungs and the liver; hepatic fatty acid composition was assessed by GC–MS. (3) Results: The old mice showed a distinct potential for endotoxin tolerance as suggested by the serum cytokine levels and gene expression in the lung tissue. Endotoxin tolerance was less pronounced in the livers of the aged mice. However, the fatty acid composition strongly differed in the liver tissues of the young and old mice with a distinct change in the ratio of C18 to C16 fatty acids. (4) Conclusions: Endotoxin tolerance is maintained in advanced age, but changes in the metabolic tissue homeostasis may lead to an altered immune response in old individuals.
DOI of the first publication: 10.3390/biology12040530
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-396309
ISSN: 2079-7737
Date of registration: 2-May-2023
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Sport- und Präventivmedizin
NT - Pharmazie
Professorship: M - Prof. Dr. Tim Meyer
NT - Prof. Dr. Alexandra K. Kiemer
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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