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Titel: Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling
VerfasserIn: Beiersdorfer, Antonia
Scheller, Anja
Kirchhoff, Frank
Lohr, Christian
Sprache: Englisch
Titel: Glia
Bandnummer: 67
Heft: 7
Seiten: 1385-1400
Verlag/Plattform: Wiley
Erscheinungsjahr: 2019
Freie Schlagwörter: astrocyte
calcium signaling
gap junction
neurovascular coupling
olfactory ensheathing cell
panglial network
syncytium
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Astrocytes are arranged in highly organized gap junction-coupled networks, communicating via the propagation of Ca2+ waves. Astrocytes are gap junction-coupled not only to neighboring astrocytes, but also to oligodendrocytes, forming so-called panglial syncytia. It is not known, however, whether glial cells in panglial syncytia transmit information using Ca2+ signaling. We used confocal Ca2+ imaging to study intercellular communication between astrocytes and olfactory ensheathing glial cells (OECs) in in-toto preparations of the mouse olfactory bulb. Our results demonstrate that Ca2+ transients in juxtaglomerular astrocytes, evoked by local photolysis of “caged” ATP and “caged” tACPD, led to subsequent Ca2+ responses in OECs. This transmission of Ca2+ responses from astrocytes to OECs persisted in the presence of neuronal inhibition, but was absent when gap junctional coupling was suppressed with carbenoxolone. When Ca2+ transients were directly evoked in OECs by puff application of DHPG, they resulted in delayed Ca2+ responses in juxtaglomerular astrocytes, indicating that panglial transmission of Ca2+ signals occurred in a bidirectional manner. In addition, panglial transmission of Ca2+ signals from astrocytes to OECs resulted in vasoconstriction of OEC-associated blood vessels in the olfactory nerve layer. Our results demonstrate functional transmission of Ca2+ signals between different classes of glial cells within gap junction-coupled panglial networks and the resulting regulation of blood vessel diameter in the olfactory bulb.
DOI der Erstveröffentlichung: 10.1002/glia.23613
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/10.1002/glia.23613
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-395519
hdl:20.500.11880/35647
http://dx.doi.org/10.22028/D291-39551
ISSN: 1098-1136
0894-1491
Datum des Eintrags: 17-Apr-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23613&file=glia23613-sup-0001-supinfo.docx
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23613&file=glia23613-sup-0002-Movie1.avi
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Physiologie
Professur: M - Prof. Dr. Frank Kirchhoff
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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