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doi:10.22028/D291-39551
Titel: | Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling |
VerfasserIn: | Beiersdorfer, Antonia Scheller, Anja Kirchhoff, Frank Lohr, Christian |
Sprache: | Englisch |
Titel: | Glia |
Bandnummer: | 67 |
Heft: | 7 |
Seiten: | 1385-1400 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2019 |
Freie Schlagwörter: | astrocyte calcium signaling gap junction neurovascular coupling olfactory ensheathing cell panglial network syncytium |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Astrocytes are arranged in highly organized gap junction-coupled networks, communicating via the propagation of Ca2+ waves. Astrocytes are gap junction-coupled not only to neighboring astrocytes, but also to oligodendrocytes, forming so-called panglial syncytia. It is not known, however, whether glial cells in panglial syncytia transmit information using Ca2+ signaling. We used confocal Ca2+ imaging to study intercellular communication between astrocytes and olfactory ensheathing glial cells (OECs) in in-toto preparations of the mouse olfactory bulb. Our results demonstrate that Ca2+ transients in juxtaglomerular astrocytes, evoked by local photolysis of “caged” ATP and “caged” tACPD, led to subsequent Ca2+ responses in OECs. This transmission of Ca2+ responses from astrocytes to OECs persisted in the presence of neuronal inhibition, but was absent when gap junctional coupling was suppressed with carbenoxolone. When Ca2+ transients were directly evoked in OECs by puff application of DHPG, they resulted in delayed Ca2+ responses in juxtaglomerular astrocytes, indicating that panglial transmission of Ca2+ signals occurred in a bidirectional manner. In addition, panglial transmission of Ca2+ signals from astrocytes to OECs resulted in vasoconstriction of OEC-associated blood vessels in the olfactory nerve layer. Our results demonstrate functional transmission of Ca2+ signals between different classes of glial cells within gap junction-coupled panglial networks and the resulting regulation of blood vessel diameter in the olfactory bulb. |
DOI der Erstveröffentlichung: | 10.1002/glia.23613 |
URL der Erstveröffentlichung: | https://onlinelibrary.wiley.com/doi/10.1002/glia.23613 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-395519 hdl:20.500.11880/35647 http://dx.doi.org/10.22028/D291-39551 |
ISSN: | 1098-1136 0894-1491 |
Datum des Eintrags: | 17-Apr-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23613&file=glia23613-sup-0001-supinfo.docx https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23613&file=glia23613-sup-0002-Movie1.avi |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Physiologie |
Professur: | M - Prof. Dr. Frank Kirchhoff |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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