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Titel: Early embryonic NG2 glia are exclusively gliogenic and do not generate neurons in the brain
VerfasserIn: Huang, Wenhui UdsID
Guo, Qilin
Bai, Xianshu UdsID
Scheller, Anja UdsID
Kirchhoff, Frank UdsID
Sprache: Englisch
In:
Titel: Glia
Bandnummer: 67
Heft: 6
Seiten: 1094-1103
Verlag/Plattform: Wiley
Erscheinungsjahr: 2019
Freie Schlagwörter: differentiation
embryonic brain
neurons
NG2 glia
oligodendrocyte lineage
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: In the central nervous system, the type I transmembrane glycoprotein NG2 (nerve-glia antigen 2) is only expressed by pericytes and oligodendrocyte precursor cells (OPCs). Therefore, OPCs are also termed NG2 glia. Their fate during development has been investigated systematically in several genetically modified mouse models. Consensus exists that postnatal NG2 glia are restricted to the oligodendrocyte (OL) lineage, while, at least in the forebrain, embryonic NG2 glia could also generate astrocytes. In addition, experimental evidence for a neurogenic potential of NG2 glia in the early embryonic brain (before E16.5) has been provided. However, this observation is still controversial. Here, we took advantage of reliable transgene expression in NG2-EYFP and NG2-CreERT2 knock-in mice to study the fate of early embryonic NG2 glia. While pericytes were the main cells with robust NG2 gene activity at E12.5, only a few OPCs expressed NG2 at this early stage of embryogenesis. Subsequently, this proportion of OPCs increased from 3% (E12.5) to 11% and 25% at E14.5 and E17.5, respectively. When Cre DNA recombinase activity was induced at E12.5 and E14.5 and pups were analyzed at postnatal day 0 (P0) and P10, the vast majority of recombined cells, besides pericytes, belonged to the OL lineage cells, with few astrocytes in the ventral forebrain. In other brain regions such as brain stem, cerebellum, and olfactory bulb only OL lineage cells were detected. Therefore, we conclude that NG2 glia from early embryonic brain are restricted to a gliogenic fate and do not differentiate into neurons after birth.
DOI der Erstveröffentlichung: 10.1002/glia.23590
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/10.1002/glia.23590
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-395509
hdl:20.500.11880/35646
http://dx.doi.org/10.22028/D291-39550
ISSN: 1098-1136
0894-1491
Datum des Eintrags: 17-Apr-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23590&file=glia23590-sup-0001-FigureS1.tif
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23590&file=glia23590-sup-0002-FigureS2.tif
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fglia.23590&file=glia23590-sup-0003-FigureS3.tif
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Physiologie
Professur: M - Prof. Dr. Frank Kirchhoff
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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