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Titel: Magnetic resonance imaging of cerebrospinal fluid outflow after low-rate lateral ventricle infusion in mice
VerfasserIn: Decker, Yann
Krämer, Jonas
Xin, Li
Müller, Andreas
Scheller, Anja
Fassbender, Klaus
Proulx, Steven T.
Sprache: Englisch
Titel: JCI insight
Bandnummer: 7 (2022)
Heft: 3
Verlag/Plattform: American Society for Clinical Investigation
Erscheinungsjahr: 2021
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The anatomical routes for the clearance of cerebrospinal fluid (CSF) remain incompletely understood. However, recent evidence has given strong support for routes leading to lymphatic vessels. A current debate centers upon the routes through which CSF can access lymphatics, with evidence emerging for either direct routes to meningeal lymphatics or along cranial nerves to reach lymphatics outside the skull. Here, a method was established to infuse contrast agent into the ventricles using indwelling cannulae during imaging of mice at 2 and 12 months of age by magnetic resonance imaging. As expected, a substantial decline in overall CSF turnover was found with aging. Quantifications demonstrated that the bulk of the contrast agent flowed from the ventricles to the subarachnoid space in the basal cisterns. Comparatively little contrast agent signal was found at the dorsal aspect of the skull. The imaging dynamics from the 2 cohorts revealed that the contrast agent was cleared from the cranium through the cribriform plate to the nasopharyngeal lymphatics. On decalcified sections, we confirmed that fluorescently labeled ovalbumin drained through the cribriform plate and could be found within lymphatics surrounding the nasopharynx. In conclusion, routes leading to nasopharyngeal lymphatics appear to be a major efflux pathway for cranial CSF.
DOI der Erstveröffentlichung: 10.1172/jci.insight.150881
URL der Erstveröffentlichung: https://insight.jci.org/articles/view/150881
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-395437
hdl:20.500.11880/35639
http://dx.doi.org/10.22028/D291-39543
ISSN: 2379-3708
Datum des Eintrags: 17-Apr-2023
Bezeichnung des in Beziehung stehenden Objekts: Supplemental material
In Beziehung stehendes Objekt: https://insight.jci.org/articles/view/150881/sd/1
https://insight.jci.org/articles/view/150881/sd/2
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Neurologie und Psychiatrie
M - Physiologie
M - Radiologie
Professur: M - Prof. Dr. Arno Bücker
M - Prof. Dr. Klaus Faßbender
M - Prof. Dr. Frank Kirchhoff
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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