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doi:10.22028/D291-39543
Titel: | Magnetic resonance imaging of cerebrospinal fluid outflow after low-rate lateral ventricle infusion in mice |
VerfasserIn: | Decker, Yann Krämer, Jonas Xin, Li Müller, Andreas Scheller, Anja Fassbender, Klaus Proulx, Steven T. |
Sprache: | Englisch |
Titel: | JCI insight |
Bandnummer: | 7 (2022) |
Heft: | 3 |
Verlag/Plattform: | American Society for Clinical Investigation |
Erscheinungsjahr: | 2021 |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The anatomical routes for the clearance of cerebrospinal fluid (CSF) remain incompletely understood. However, recent evidence has given strong support for routes leading to lymphatic vessels. A current debate centers upon the routes through which CSF can access lymphatics, with evidence emerging for either direct routes to meningeal lymphatics or along cranial nerves to reach lymphatics outside the skull. Here, a method was established to infuse contrast agent into the ventricles using indwelling cannulae during imaging of mice at 2 and 12 months of age by magnetic resonance imaging. As expected, a substantial decline in overall CSF turnover was found with aging. Quantifications demonstrated that the bulk of the contrast agent flowed from the ventricles to the subarachnoid space in the basal cisterns. Comparatively little contrast agent signal was found at the dorsal aspect of the skull. The imaging dynamics from the 2 cohorts revealed that the contrast agent was cleared from the cranium through the cribriform plate to the nasopharyngeal lymphatics. On decalcified sections, we confirmed that fluorescently labeled ovalbumin drained through the cribriform plate and could be found within lymphatics surrounding the nasopharynx. In conclusion, routes leading to nasopharyngeal lymphatics appear to be a major efflux pathway for cranial CSF. |
DOI der Erstveröffentlichung: | 10.1172/jci.insight.150881 |
URL der Erstveröffentlichung: | https://insight.jci.org/articles/view/150881 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-395437 hdl:20.500.11880/35639 http://dx.doi.org/10.22028/D291-39543 |
ISSN: | 2379-3708 |
Datum des Eintrags: | 17-Apr-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplemental material |
In Beziehung stehendes Objekt: | https://insight.jci.org/articles/view/150881/sd/1 https://insight.jci.org/articles/view/150881/sd/2 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Neurologie und Psychiatrie M - Physiologie M - Radiologie |
Professur: | M - Prof. Dr. Arno Bücker M - Prof. Dr. Klaus Faßbender M - Prof. Dr. Frank Kirchhoff |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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150881.2-20220119160358-covered-e0fd13ba177f913fd3156f593ead4cfd.pdf | 9,17 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons