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doi:10.22028/D291-39355
Titel: | Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity |
VerfasserIn: | Bhusari, Shardul Sankaran, Shrikrishnan del Campo, Aránzazu |
Sprache: | Englisch |
Titel: | Advanced Science |
Bandnummer: | 9 |
Heft: | 17 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | bacterial hydrogel bacterial–materials interactions cell encapsulation dynamic hydrogel engineered living material |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Engineered living materials (ELMs) are a new class of materials in which living organism incorporated into diffusive matrices uptake a fundamental role in material’s composition and function. Understanding how the spatial confinement in 3D can regulate the behavior of the embedded cells is crucial to design and predict ELM’s function, minimize their environmental impact and facilitate their translation into applied materials. This study investigates the growth and metabolic activity of bacteria within an associative hydrogel network (Pluronic-based) with mechanical properties that can be tuned by introducing a variable degree of acrylate crosslinks. Individual bacteria distributed in the hydrogel matrix at low density form functional colonies whose size is controlled by the extent of permanent crosslinks. With increasing stiffness and elastic response to deformation of the matrix, a decrease in colony volumes and an increase in their sphericity are observed. Protein production follows a different pattern with higher production yields occurring in networks with intermediate permanent crosslinking degrees. These results demonstrate that matrix design can be used to control and regulate the composition and function of ELMs containing microorganisms. Interestingly, design parameters for matrices to regulate bacteria behavior show similarities to those elucidated for 3D culture of mammalian cells. |
DOI der Erstveröffentlichung: | 10.1002/advs.202106026 |
URL der Erstveröffentlichung: | https://doi.org/10.1002/advs.202106026 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-393557 hdl:20.500.11880/35484 http://dx.doi.org/10.22028/D291-39355 |
ISSN: | 2198-3844 |
Datum des Eintrags: | 22-Mär-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0001-SuppMat.pdf https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0002-VideoS1.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0003-VideoS2.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0004-VideoS3.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0005-VideoS4.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0006-VideoS5.avi |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Chemie |
Professur: | NT - Prof. Dr. Aránzazu del Campo |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
Advanced Science - 2022 - Bhusari - Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity.pdf | 1,92 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons