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Titel: Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity
VerfasserIn: Bhusari, Shardul
Sankaran, Shrikrishnan
del Campo, Aránzazu
Sprache: Englisch
Titel: Advanced Science
Bandnummer: 9
Heft: 17
Verlag/Plattform: Wiley
Erscheinungsjahr: 2022
Freie Schlagwörter: bacterial hydrogel
bacterial–materials interactions
cell encapsulation
dynamic hydrogel
engineered living material
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Engineered living materials (ELMs) are a new class of materials in which living organism incorporated into diffusive matrices uptake a fundamental role in material’s composition and function. Understanding how the spatial confinement in 3D can regulate the behavior of the embedded cells is crucial to design and predict ELM’s function, minimize their environmental impact and facilitate their translation into applied materials. This study investigates the growth and metabolic activity of bacteria within an associative hydrogel network (Pluronic-based) with mechanical properties that can be tuned by introducing a variable degree of acrylate crosslinks. Individual bacteria distributed in the hydrogel matrix at low density form functional colonies whose size is controlled by the extent of permanent crosslinks. With increasing stiffness and elastic response to deformation of the matrix, a decrease in colony volumes and an increase in their sphericity are observed. Protein production follows a different pattern with higher production yields occurring in networks with intermediate permanent crosslinking degrees. These results demonstrate that matrix design can be used to control and regulate the composition and function of ELMs containing microorganisms. Interestingly, design parameters for matrices to regulate bacteria behavior show similarities to those elucidated for 3D culture of mammalian cells.
DOI der Erstveröffentlichung: 10.1002/advs.202106026
URL der Erstveröffentlichung: https://doi.org/10.1002/advs.202106026
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-393557
hdl:20.500.11880/35484
http://dx.doi.org/10.22028/D291-39355
ISSN: 2198-3844
Datum des Eintrags: 22-Mär-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadvs.202106026&file=advs3890-sup-0001-SuppMat.pdf
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Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons