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doi:10.22028/D291-39353
Titel: | Gelation Kinetics and Mechanical Properties of Thiol-Tetrazole Methylsulfone Hydrogels Designed for Cell Encapsulation |
VerfasserIn: | de Miguel-Jiménez, Adrián Ebeling, Bastian Paez, Julieta I. Fink-Straube, Claudia Pearson, Samuel del Campo, Aránzazu |
Sprache: | Englisch |
Titel: | Macromolecular Bioscience |
Bandnummer: | 23 (2023) |
Heft: | 2 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | cell culture cell encapsulation crosslinking gelation hydrogel |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Hydrogel precursors that crosslink within minutes are essential for the development of cell encapsulation matrices and their implementation in automated systems. Such timescales allow sufficient mixing of cells and hydrogel precursors under low shear forces and the achievement of homogeneous networks and cell distributions in the 3D cell culture. The previous work showed that the thiol-tetrazole methylsulfone (TzMS) reaction crosslinks star-poly(ethylene glycol) (PEG) hydrogels within minutes at around physiological pH and can be accelerated or slowed down with small pH changes. The resulting hydrogels are cytocompatible and stable in cell culture conditions. Here, the gelation kinetics and mechanical properties of PEG-based hydrogels formed by thiol-TzMS crosslinking as a function of buffer, crosslinker structure and degree of TzMS functionality are reported. Crosslinkers of different architecture, length and chemical nature (PEG versus peptide) are tested, and degree of TzMS functionality is modified by inclusion of RGD cell-adhesive ligand, all at concentration ranges typically used in cell culture. These studies corroborate that thiol/PEG-4TzMS hydrogels show gelation times and stiffnesses that are suitable for 3D cell encapsulation and tunable through changes in hydrogel composition. The results of this study guide formulation of encapsulating hydrogels for manual and automated 3D cell culture. |
DOI der Erstveröffentlichung: | 10.1002/mabi.202200419 |
URL der Erstveröffentlichung: | https://doi.org/10.1002/mabi.202200419 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-393535 hdl:20.500.11880/35482 http://dx.doi.org/10.22028/D291-39353 |
ISSN: | 1616-5195 1616-5187 |
Datum des Eintrags: | 22-Mär-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fmabi.202200419&file=mabi202200419-sup-0001-SuppMat.pdf |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Chemie |
Professur: | NT - Prof. Dr. Aránzazu del Campo |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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Macromolecular Bioscience - 2022 - Miguel‐Jim nez - Gelation Kinetics and Mechanical Properties of Thiol‐Tetrazole.pdf | 1,5 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons