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Titel: Gelation Kinetics and Mechanical Properties of Thiol-Tetrazole Methylsulfone Hydrogels Designed for Cell Encapsulation
VerfasserIn: de Miguel-Jiménez, Adrián
Ebeling, Bastian
Paez, Julieta I.
Fink-Straube, Claudia
Pearson, Samuel
del Campo, Aránzazu
Sprache: Englisch
Titel: Macromolecular Bioscience
Bandnummer: 23 (2023)
Heft: 2
Verlag/Plattform: Wiley
Erscheinungsjahr: 2022
Freie Schlagwörter: cell culture
cell encapsulation
crosslinking
gelation
hydrogel
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Hydrogel precursors that crosslink within minutes are essential for the development of cell encapsulation matrices and their implementation in automated systems. Such timescales allow sufficient mixing of cells and hydrogel precursors under low shear forces and the achievement of homogeneous networks and cell distributions in the 3D cell culture. The previous work showed that the thiol-tetrazole methylsulfone (TzMS) reaction crosslinks star-poly(ethylene glycol) (PEG) hydrogels within minutes at around physiological pH and can be accelerated or slowed down with small pH changes. The resulting hydrogels are cytocompatible and stable in cell culture conditions. Here, the gelation kinetics and mechanical properties of PEG-based hydrogels formed by thiol-TzMS crosslinking as a function of buffer, crosslinker structure and degree of TzMS functionality are reported. Crosslinkers of different architecture, length and chemical nature (PEG versus peptide) are tested, and degree of TzMS functionality is modified by inclusion of RGD cell-adhesive ligand, all at concentration ranges typically used in cell culture. These studies corroborate that thiol/PEG-4TzMS hydrogels show gelation times and stiffnesses that are suitable for 3D cell encapsulation and tunable through changes in hydrogel composition. The results of this study guide formulation of encapsulating hydrogels for manual and automated 3D cell culture.
DOI der Erstveröffentlichung: 10.1002/mabi.202200419
URL der Erstveröffentlichung: https://doi.org/10.1002/mabi.202200419
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-393535
hdl:20.500.11880/35482
http://dx.doi.org/10.22028/D291-39353
ISSN: 1616-5195
1616-5187
Datum des Eintrags: 22-Mär-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fmabi.202200419&file=mabi202200419-sup-0001-SuppMat.pdf
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons