Please use this identifier to cite or link to this item: doi:10.22028/D291-39219
Title: Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion
Author(s): Meiers, Joscha
Dastbaz, Jan
Adam, Sebastian
Rasheed, Sari
Kirsch, Susanne H.
Meiser, Peter
Gross, Peter
Müller, Rolf
Titz, Alexander
Language: English
Title: ChemBioChem
Volume: 24 (2023)
Issue: 3
Publisher/Platform: Wiley
Year of Publication: 2022
Free key words: bromelain
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar KD in a carbohydrate-dependent fashion.
DOI of the first publication: 10.1002/cbic.202200463
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-392190
ISSN: 1439-7633
Date of registration: 6-Mar-2023
Description of the related object: Supporting Information
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
NT - Pharmazie
Professorship: NT - Prof. Dr. Rolf Müller
NT - Univ.-Prof. Dr. phil. Alexander Titz
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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