Please use this identifier to cite or link to this item: doi:10.22028/D291-39207
Title: Effects of long-term immobilisation on endomysium of the soleus muscle in humans
Author(s): Thot, Georgina K.
Berwanger, Carolin
Mulder, Edwin
Lee, Jessica K.
Lichterfeld, Yannick
Ganse, Bergita
Giakoumaki, Ifigeneia
Degens, Hans
Duran, Ibrahim
Schönau, Eckhard
Clemen, Christoph S.
Brachvogel, Bent
Rittweger, Jörn
Language: English
Title: Experimental Physiology
Volume: 106
Issue: 10
Pages: 2038-2045
Publisher/Platform: Wiley
Year of Publication: 2021
Free key words: connective tissue
muscle atrophy
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Muscle fibres atrophy during conditions of disuse. Whilst animal data suggest an increase in endomysium content with disuse, that information is not available for humans. We hypothesised that endomysium content increases during immobilisation. To test this hypothesis, biopsy samples of the soleus muscle obtained from 21 volunteers who underwent 60 days of bed rest were analysed using immunofluorescence-labelled laminin γ-1 to delineate individual muscle fibres as well as the endomysium space. The endomysium-to-fibre-area ratio (EFAr, as a percentage) was assessed as a measure related to stiffness, and the endomysium-tofibre-number ratio (EFNr) was calculated to determine whether any increase in EFAr was absolute, or could be attributed to muscle fibre shrinkage. As expected, we found muscle fibre atrophy (P = 0.0031) that amounted to shrinkage by 16.6% (SD 28.2%) on day 55 of bed rest. ENAr increased on day 55 of bed rest (P < 0.001). However, when analysing EFNr, no effect of bed rest was found (P = 0.62). These results demonstrate that an increase in EFAr is likely to be a direct effect of muscle fibre atrophy. Based on the assumption that the total number of muscle fibres remains unchanged during 55 days of bed rest, this implies that the absolute amount of connective tissue in the soleus muscle remained unchanged. The increased relative endomysium content, however, could be functionally related to an increase in muscle stiffness.
DOI of the first publication: 10.1113/EP089734
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-392076
ISSN: 1469-445X
Date of registration: 3-Mar-2023
Description of the related object: Supporting Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. med. Bergita Ganse
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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