Please use this identifier to cite or link to this item: doi:10.22028/D291-39206
Title: Local Application of Mineral-Coated Microparticles Loaded With VEGF and BMP-2 Induces the Healing of Murine Atrophic Non-Unions
Author(s): Orth, M.
Fritz, T.
Stutz, J.
Scheuer, C.
Ganse, B.
Bullinger, Y.
Lee, J. S.
Murphy, W. L.
Laschke, M. W.
Menger, M. D.
Pohlemann, T.
Language: English
Title: Frontiers in Bioengineering and Biotechnology
Volume: 9
Publisher/Platform: Frontiers
Year of Publication: 2022
Free key words: non-union
mineral-coated microparticles
bone healing
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Deficient angiogenesis and disturbed osteogenesis are key factors for the development of nonunions. Mineral-coated microparticles (MCM) represent a sophisticated carrier system for the delivery of vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP)-2. In this study, we investigated whether a combination of VEGF- and BMP2-loaded MCM (MCM + VB) with a ratio of 1:2 improves bone repair in non-unions. For this purpose, we applied MCM + VB or unloaded MCM in a murine non-union model and studied the process of bone healing by means of radiological, biomechanical, histomorphometric, immunohistochemical and Western blot techniques after 14 and 70 days. MCM-free non-unions served as controls. Bone defects treated with MCM + VB exhibited osseous bridging, an improved biomechanical stiffness, an increased bone volume within the callus including ongoing mineralization, increased vascularization, and a histologically larger total periosteal callus area consisting predominantly of osseous tissue when compared to defects of the other groups. Western blot analyses on day 14 revealed a higher expression of osteoprotegerin (OPG) and vice versa reduced expression of receptor activator of NF-κB ligand (RANKL) in bone defects treated with MCM + VB. On day 70, these defects exhibited an increased expression of erythropoietin (EPO), EPOreceptor and BMP-4. These findings indicate that the use of MCM for spatiotemporal controlled delivery of VEGF and BMP-2 shows great potential to improve bone healing in atrophic non-unions by promoting angiogenesis and osteogenesis as well as reducing early osteoclast activity.
DOI of the first publication: 10.3389/fbioe.2021.809397
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-392064
ISSN: 2296-4185
Date of registration: 3-Mar-2023
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
M - Neurochirurgie
Professorship: M - Prof. Dr. med. Bergita Ganse
M - Prof. Dr. Michael D. Menger
M - Prof. Dr. Joachim Oertel
M - Prof. Dr. Tim Pohlemann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
fbioe-09-809397.pdf4,06 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons