Please use this identifier to cite or link to this item: doi:10.22028/D291-34881
Title: Discovery and Genetic Characterization of Novel Paramyxoviruses Related to the Genus Henipavirus in Crocidura Species in the Republic of Korea
Author(s): Lee, Seung-Ho
Kim, Kijin
Kim, Jongwoo
No, Jin Sun
Park, Kyungmin
Budhathoki, Shailesh
Lee, Seung Ho
Lee, Jingyeong
Cho, Seung Hye
Cho, Seungchan
Lee, Geum-Young
Hwang, Jusun
Kim, Heung-Chul
Klein, Terry A.
Uhm, Chang-Sub
Kim, Won-Keun
Song, Jin-Won
Language: English
Title: Viruses
Volume: 13
Issue: 10
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: Crocidura paramyxovirus
novel virus discovery
next-generation sequencing
genetic characterization and diversity
potential zoonosis
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were examined for the prevalence of paramyxovirus infections. Paramyxovirus RNA was observed in 26 (22.6%) shrews collected at five trapping sites, Republic of Korea. Herein, we report two genetically distinct novel paramyxoviruses (genus: Henipavirus): Gamak virus (GAKV) and Daeryong virus (DARV) isolated from C. lasiura and C. shantungensis, respectively. Two GAKVs and one DARV were nearly completely sequenced using next-generation sequencing. GAKV and DARV contain six genes (30 -N-P-M-F-G-L-50 ) with genome sizes of 18,460 nucleotides and 19,471 nucleotides, respectively. The phylogenetic inference demonstrated that GAKV and DARV form independent genetic lineages of Henipavirus in Crocidura species. GAKV-infected human lung epithelial cells elicited the induction of type I/III interferons, interferon-stimulated genes, and proinflammatory cytokines. In conclusion, this study contributes further understandings of the molecular prevalence, genetic characteristics and diversity, and zoonotic potential of novel paramyxoviruses in shrews.
DOI of the first publication: 10.3390/v13102020
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-348814
ISSN: 1999-4915
Date of registration: 28-Feb-2023
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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