Please use this identifier to cite or link to this item: doi:10.22028/D291-38914
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Title: Phospholipids diffusion on the surface of model lipid droplets
Author(s): Asfia, Shima
Seemann, Ralf
Fleury, Jean-Baptiste
Language: English
Title: Biochimica et Biophysica Acta (BBA) : Biomembranes
Volume: 1865
Issue: 1
Publisher/Platform: Elsevier
Year of Publication: 2023
Free key words: Phospholipid
Lipid droplet
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Lipid droplets (LD) are organelles localized in the membrane of the Endoplasmic Reticulum (ER) that play an important role in metabolic functions. They consist of a core of neutral lipids surrounded by a monolayer of phosphoplipids and proteins resembling an oil-in-water emulsion droplet. Many studies have focused on the biophysical properties of these LDs. However, despite numerous efforts, we are lacking information on the mobility of phospholipids on the LDs surface, although they may play a key role in the protein distribution. In this article, we developed a microfluidic setup that allows the formation of a triolein–buffer interface decorated with a phospholipid monolayer. Using this setup, we measured the motility of phospholipid molecules by performing Fluorescent Recovery After Photobleaching (FRAP) experiments for different lipidic compositions. The results of the FRAP measurements reveal that the motility of phospholipids is controlled by the monolayer packing decorating the interface.
DOI of the first publication: 10.1016/j.bbamem.2022.184074
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-389148
ISSN: 0005-2736
Date of registration: 6-Feb-2023
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Ralf Seemann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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