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doi:10.22028/D291-38907
Titel: | Effect of Poly(Oxanorbonene)- and Poly(Methacrylate)-Based Polyzwitterionic Surface Coatings on Cell Adhesion and Gene Expression of Human Keratinocytes |
VerfasserIn: | Eickenscheidt, Alice Lavaux, Valentine Paschke, Stefan Martínez, Alejandro Guajardo Schönemann, Eric Laschewsky, André Lienkamp, Karen Staszewski, Ori |
Sprache: | Englisch |
Titel: | Macromolecular Bioscience |
Bandnummer: | 22 |
Heft: | 11 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | cell compatibility focal adhesion gene expression immune response polyzwitterions |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Polyzwitterions are generally known for their anti-adhesive properties, including resistance to protein and cell adhesion, and overall high bio-inertness. Yet there are a few polyzwitterions to which mammalian cells do adhere. To understand the structural features of this behavior, a panel of polyzwitterions with different functional groups and overall degrees of hydrophobicity is analyzed here, and their physical and biological properties are correlated to these structural differences. Cell adhesion is focused on, which is the basic requirement for cell viability, proliferation, and growth. With the here presented polyzwitterion panel, three different types of cell-surface interactions are observed: adhesion, slight attachment, and cell repellency. Using immunofluorescence methods, it is found that human keratinocytes (HaCaT) form focal adhesions on the cell-adhesive polyzwitterions, but not on the sample that has only slight cell attachment. Gene expression analysis indicates that HaCaT cells cultivated in the presence of a non-adhesive polyzwitterion have up-regulated inflammatory and apoptosis-related cell signaling pathways, while the gene expression of HaCaT cells grown on a cell-adhesive polyzwitterion does not differ from the gene expression of the growth control, and thus can be defined as fully cell-compatible. |
DOI der Erstveröffentlichung: | 10.1002/mabi.202200225 |
URL der Erstveröffentlichung: | https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200225 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-389070 hdl:20.500.11880/35103 http://dx.doi.org/10.22028/D291-38907 |
ISSN: | 1616-5195 1616-5187 |
Datum des Eintrags: | 3-Feb-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fmabi.202200225&file=mabi202200225-sup-0001-SuppMat.pdf |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Materialwissenschaft und Werkstofftechnik |
Professur: | NT - Dr. Karen Lienkamp |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
Macromolecular Bioscience - 2022 - Eickenscheidt - Effect of Poly Oxanorbonene ‐ and Poly Methacrylate ‐Based.pdf | 3,02 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons