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Titel: Effect of Poly(Oxanorbonene)- and Poly(Methacrylate)-Based Polyzwitterionic Surface Coatings on Cell Adhesion and Gene Expression of Human Keratinocytes
VerfasserIn: Eickenscheidt, Alice
Lavaux, Valentine
Paschke, Stefan
Martínez, Alejandro Guajardo
Schönemann, Eric
Laschewsky, André
Lienkamp, Karen
Staszewski, Ori
Sprache: Englisch
Titel: Macromolecular Bioscience
Bandnummer: 22
Heft: 11
Verlag/Plattform: Wiley
Erscheinungsjahr: 2022
Freie Schlagwörter: cell compatibility
focal adhesion
gene expression
immune response
polyzwitterions
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Polyzwitterions are generally known for their anti-adhesive properties, including resistance to protein and cell adhesion, and overall high bio-inertness. Yet there are a few polyzwitterions to which mammalian cells do adhere. To understand the structural features of this behavior, a panel of polyzwitterions with different functional groups and overall degrees of hydrophobicity is analyzed here, and their physical and biological properties are correlated to these structural differences. Cell adhesion is focused on, which is the basic requirement for cell viability, proliferation, and growth. With the here presented polyzwitterion panel, three different types of cell-surface interactions are observed: adhesion, slight attachment, and cell repellency. Using immunofluorescence methods, it is found that human keratinocytes (HaCaT) form focal adhesions on the cell-adhesive polyzwitterions, but not on the sample that has only slight cell attachment. Gene expression analysis indicates that HaCaT cells cultivated in the presence of a non-adhesive polyzwitterion have up-regulated inflammatory and apoptosis-related cell signaling pathways, while the gene expression of HaCaT cells grown on a cell-adhesive polyzwitterion does not differ from the gene expression of the growth control, and thus can be defined as fully cell-compatible.
DOI der Erstveröffentlichung: 10.1002/mabi.202200225
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200225
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-389070
hdl:20.500.11880/35103
http://dx.doi.org/10.22028/D291-38907
ISSN: 1616-5195
1616-5187
Datum des Eintrags: 3-Feb-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fmabi.202200225&file=mabi202200225-sup-0001-SuppMat.pdf
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Materialwissenschaft und Werkstofftechnik
Professur: NT - Dr. Karen Lienkamp
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons