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doi:10.22028/D291-38792
Titel: | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
VerfasserIn: | Schnabel, Isabelle Schneitler, Sophie Schüttoff, Tom Trawinski, Henning Lübbert, Christoph Jassoy, Christian |
Sprache: | Englisch |
Titel: | Tropical Medicine and Infectious Disease |
Bandnummer: | 8 (2023) |
Heft: | 1 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | dengue virus flavivirus ELISA antibody cross-reaction diagnostic specificity |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Dengue virus (DENV) antibody assays frequently cross-react with sera from individuals who have been infected with or vaccinated against related flaviviruses. The goal of this study was to determine the specificity of two DENV ELISAs with sera from individuals vaccinated against yellow fever virus (YFV) and Japanese encephalitis virus (JEV). The Panbio and the Novatec Dengue IgG ELISAs were tested with sera obtained 3–4 weeks or 0.5–6 years after YFV or JEV vaccination and the diagnostic specificity of the assays was determined. As controls, the sera were tested using DENV, YFV, JEV, Zika and West Nile virus neutralization assays. The diagnostic specificity of the Panbio and the Novatec ELISA with sera from YFV-vaccinated subjects was 98.2% and 88.2%, respectively. Cross-reactions were rare in the first 4 weeks despite high YFV-neutralizing antibody titers and were mostly found later. The specificity of the Panbio and Novatec assays with sera from JEV-vaccinated individuals was 100% and 92.9%. Cross-reactions occurred in the early time period after vaccination. The measurement values of the two ELISAs correlated strongly. Thus, the Panbio ELISA showed higher diagnostic specificity and may be suitable for seroprevalence studies in areas with high disease prevalence. |
DOI der Erstveröffentlichung: | 10.3390/tropicalmed8010007 |
URL der Erstveröffentlichung: | https://doi.org/10.3390/tropicalmed8010007 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-387929 hdl:20.500.11880/34976 http://dx.doi.org/10.22028/D291-38792 |
ISSN: | 2414-6366 |
Datum des Eintrags: | 23-Jan-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Materials |
In Beziehung stehendes Objekt: | https://www.mdpi.com/article/10.3390/tropicalmed8010007/s1 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Innere Medizin |
Professur: | M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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tropicalmed-08-00007-v2.pdf | 4,47 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons