Please use this identifier to cite or link to this item: doi:10.22028/D291-38789
Title: The Effect of Elasticity of Gelatin Nanoparticles on the Interaction with Macrophages
Author(s): Yildirim, Metin
Weiss, Agnes-Valencia
Schneider, Marc
Language: English
Title: Pharmaceutics
Volume: 15
Issue: 1
Publisher/Platform: MDPI
Year of Publication: 2023
Free key words: nanotechnology
drug delivery
atomic force microscopy
young’s modulus
cell uptake
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Gelatin is a biocompatible, biodegradable, cheap, and nontoxic material, which is already used for pharmaceutical applications. Nanoparticles from gelatin (GNPs) are considered a promising delivery system for hydrophilic and macromolecular drugs. Mechanical properties of particles are recognized as an important parameter affecting drug carrier interaction with biological systems. GNPs offer the preparation of particles with different stiffness. GNPs were loaded with Fluorescein isothiocyanate-labeled 150 kDa dextran (FITC-dextran) yielding also different elastic properties. GNPs were visualized using atomic force microscopy (AFM), and force–distance curves from the center of the particles were evaluated for Young’s modulus calculation. The prepared GNPs have Young’s moduli from 4.12 MPa for soft to 9.8 MPa for stiff particles. Furthermore, cytokine release (IL-6 and TNF-α), cell viability, and cell uptake were determined on macrophage cell lines from mouse (RAW 264.7) and human (dTHP-1 cells, differentiated human monocytic THP-1 cells) origin for soft and stiff GNPs. Both particle types showed good cell compatibility and did not induce IL-6 and TNF-α release from RAW 264.7 and dTHP-1 cells. Stiffer GNPs were internalized into cells faster and to a larger extent.
DOI of the first publication: 10.3390/pharmaceutics15010199
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-387894
ISSN: 1999-4923
Date of registration: 23-Jan-2023
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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