Please use this identifier to cite or link to this item: doi:10.22028/D291-38788
Title: Elderly with Varying Extents of Cardiac Disease Show Interindividual Fluctuating Myocardial TRPC6-Immunoreactivity
Author(s): Federspiel, Jan Michael
Gartner, Jil
Lipp, Peter
Schmidt, Peter
Tschernig, Thomas
Language: English
Title: Journal of Cardiovascular Development and Disease
Volume: 10
Issue: 1
Publisher/Platform: MDPI
Year of Publication: 2023
Free key words: heart
heart disease
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Both particular myocardial locations in the human heart and the canonical transient receptor potential 6 (TRPC6) cation channel have been linked with cardiac pathophysiologies. Thus, the present study mapped TRPC6-protein distribution in select anatomic locations associated with cardiac disease in the context of an orienting pathological assessment. Specimens were obtained from 5 body donors (4 formalin fixation, 1 nitrite pickling salt-ethanol-polyethylene glycol (NEP) fixation; median age 81 years; 2 females) and procured for basic histological stains and TRPC6- immunohistochemistry. The latter was analyzed descriptively regarding distribution and intensity of positive signals. The percentage of positively labelled myocardium was also determined (optical threshold method). Exclusively exploratory statistical analyses were performed. TRPC6-protein was distributed widespread and homogenously within each analyzed sample. TRPC6-immunoreactive myocardial area was comparable regarding the different anatomic regions and sex. A significantly larger area of TRPC6-immunoreactive myocardium was found in the NEP-fixed donor compared to the formalin fixed donors. Two donors with more severe heart disease showed smaller areas of myocardial TRPC6-immunoreactivity overall compared to the other 3 donors. In summary, in the elderly, TRPC6-protein is widely and homogenously distributed, and severe cardiac disease might be associated with less TRPC6-immunoreactive myocardial area. The tissue fixation method represents a potential confounder.
DOI of the first publication: 10.3390/jcdd10010026
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-387883
ISSN: 2308-3425
Date of registration: 23-Jan-2023
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Anatomie und Zellbiologie
M - Chirurgie
Professorship: M - Prof. Dr. Peter Lipp
M - Prof. Dr. Carola Meier
M - Prof. Dr. Hans Joachim Schäfers
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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