Please use this identifier to cite or link to this item: doi:10.22028/D291-38623
Title: Flavacol and Its Novel Derivative 3-β-Hydroxy Flavacol from Streptomyces sp. Pv 4-95 after the Expression of Heterologous AdpA
Author(s): Tistechok, Stepan
Stierhof, Marc
Kachor, Anna
Myronovskyi, Maksym
Gromyko, Oleksandr
Luzhetskyy, Andriy
Language: English
Title: Microorganisms
Volume: 10
Issue: 12
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: flavacol
3-β-hydroxy flavacol
secondary metabolites
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Actinomycetes are one of the main producers of biologically active compounds. However, their capabilities have not been fully evaluated due to the presence of many unexpressed silent clusters; moreover, actinomycetes can probably produce new or previously discovered natural products under certain conditions. Overexpressing the adpA gene into streptomycetes strains can unlock silent biosynthetic gene clusters. Herein, we showed that by applying this approach to Streptomyces sp. Pv 4-95 isolated from Phyllostachys viridiglaucescens rhizosphere soil, two new mass peaks were identified. NMR structure analysis identified these compounds as flavacol and a new 3-β-hydroxy flavacol derivative. We suggest that the presence of heterologous AdpA has no direct effect on the synthesis of flavacol and its derivatives in the Pv 4-95 strain. However, AdpA affects the synthesis of precursors by increasing their quantity, which then condenses into the resulting compounds.
DOI of the first publication: 10.3390/microorganisms10122335
Link to this record: urn:nbn:de:bsz:291--ds-386237
ISSN: 2076-2607
Date of registration: 23-Dec-2022
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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