Please use this identifier to cite or link to this item: doi:10.22028/D291-38618
Title: Daniellia oliveri (Rolfe) Hutch and Dalziel : Antimicrobial Activities, Cytotoxicity Evaluation, and Phytochemical Identification by GC-MS
Author(s): Tittikpina, Nassifatou Koko
Kirsch, Gilbert
Duval, Raphaël Emmanuel
Chaimbault, Patrick
Jacob, Claus
Language: English
Title: Antibiotics
Volume: 11
Issue: 12
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: Daniellia oliveri
antibacterial activity
cytotoxicity activity
phytochemical identification
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: During a previous study that identified plants used in traditional medicine in Togo to treat infectious diseases, Daniellia oliveri was specifically reported to treat intertrigo and candidiasis. Consequently, to explore the anti-infective potential of this plant, we investigated the antibacterial and the antifungal activity of the plant’s parts, as well as the cytotoxic activities of raw extracts and subsequent fractions, and the chemical composition of the most active fractions. In order to evaluate the antimicrobial activity, MICs were determined using the broth dilution method. Then, the most active fractions were evaluated for cytotoxicity by using normal human cells (MRC-5 cells) via the MTT assay. Finally, the most active and not toxic fractions were phytochemically investigated by GC-MS. Interestingly, all the raw extracts and fractions were active against the bacteria tested, with MICs ranging from 16 µg/mL to 256 µg/mL, while no antifungal activity was observed at 256 µg/mL, the highest tested concentration. Moreover, no toxicity was observed with most of the active fractions. The subsequent chemical investigation of the most interesting fractions led to identifying terpenes, phytosterols, phenolic compounds, and fatty acids as the main compounds. In conclusion, this study demonstrated that D. oliveri possesses valuable antibacterial activities in accordance with traditional use.
DOI of the first publication: 10.3390/antibiotics11121699
Link to this record: urn:nbn:de:bsz:291--ds-386180
ISSN: 2079-6382
Date of registration: 23-Dec-2022
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Claus Jacob
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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