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Titel: Melt Electrowriting of Graded Porous Scaffolds to Mimic the Matrix Structure of the Human Trabecular Meshwork
VerfasserIn: Włodarczyk-Biegun, Małgorzata K.
Villiou, Maria
Koch, Marcus
Muth, Christina
Wang, Peixi
Ott, Jenna
del Campo Bécares, Aránzazu
Sprache: Englisch
Titel: ACS biomaterials science & engineering
Bandnummer: 8
Heft: 9
Seiten: 3899-3911
Verlag/Plattform: ACS
Erscheinungsjahr: 2022
Freie Schlagwörter: melt electrowriting
human trabecular meshwork
glaucoma
3D printing
poly(caprolactone)
tissue engineering
DDC-Sachgruppe: 540 Chemie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The permeability of the human trabecular meshwork (HTM) regulates eye pressure via a porosity gradient across its thickness modulated by stacked layers of matrix fibrils and cells. Changes in HTM porosity are associated with increases in intraocular pressure and the progress of diseases such as glaucoma. Engineered HTMs could help to understand the structure–function relation in natural tissues and lead to new regenerative solutions. Here, melt electrowriting (MEW) is explored as a biofabrication technique to produce fibrillar, porous scaffolds that mimic the multilayer, gradient structure of native HTM. Poly(caprolactone) constructs with a height of 125–500 μm and fiber diameters of 10–12 μm are printed. Scaffolds with a tensile modulus between 5.6 and 13 MPa and a static compression modulus in the range of 6–360 kPa are obtained by varying the scaffold design, that is, the density and orientation of the fibers and number of stacked layers. Primary HTM cells attach to the scaffolds, proliferate, and form a confluent layer within 8–14 days, depending on the scaffold design. High cell viability and cell morphology close to that in the native tissue are observed. The present work demonstrates the utility of MEW for reconstructing complex morphological features of natural tissues.
DOI der Erstveröffentlichung: 10.1021/acsbiomaterials.2c00623
URL der Erstveröffentlichung: https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00623
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-374978
hdl:20.500.11880/34792
http://dx.doi.org/10.22028/D291-37497
ISSN: 2373-9878
Datum des Eintrags: 20-Dez-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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