Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-37497
Titel: | Melt Electrowriting of Graded Porous Scaffolds to Mimic the Matrix Structure of the Human Trabecular Meshwork |
VerfasserIn: | Włodarczyk-Biegun, Małgorzata K. Villiou, Maria Koch, Marcus Muth, Christina Wang, Peixi Ott, Jenna del Campo Bécares, Aránzazu |
Sprache: | Englisch |
Titel: | ACS biomaterials science & engineering |
Bandnummer: | 8 |
Heft: | 9 |
Seiten: | 3899-3911 |
Verlag/Plattform: | ACS |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | melt electrowriting human trabecular meshwork glaucoma 3D printing poly(caprolactone) tissue engineering |
DDC-Sachgruppe: | 540 Chemie |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The permeability of the human trabecular meshwork (HTM) regulates eye pressure via a porosity gradient across its thickness modulated by stacked layers of matrix fibrils and cells. Changes in HTM porosity are associated with increases in intraocular pressure and the progress of diseases such as glaucoma. Engineered HTMs could help to understand the structure–function relation in natural tissues and lead to new regenerative solutions. Here, melt electrowriting (MEW) is explored as a biofabrication technique to produce fibrillar, porous scaffolds that mimic the multilayer, gradient structure of native HTM. Poly(caprolactone) constructs with a height of 125–500 μm and fiber diameters of 10–12 μm are printed. Scaffolds with a tensile modulus between 5.6 and 13 MPa and a static compression modulus in the range of 6–360 kPa are obtained by varying the scaffold design, that is, the density and orientation of the fibers and number of stacked layers. Primary HTM cells attach to the scaffolds, proliferate, and form a confluent layer within 8–14 days, depending on the scaffold design. High cell viability and cell morphology close to that in the native tissue are observed. The present work demonstrates the utility of MEW for reconstructing complex morphological features of natural tissues. |
DOI der Erstveröffentlichung: | 10.1021/acsbiomaterials.2c00623 |
URL der Erstveröffentlichung: | https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00623 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-374978 hdl:20.500.11880/34792 http://dx.doi.org/10.22028/D291-37497 |
ISSN: | 2373-9878 |
Datum des Eintrags: | 20-Dez-2022 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Chemie |
Professur: | NT - Prof. Dr. Aránzazu del Campo |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.