Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-38495
Titel: | Appearance of Alveolar Macrophage Subpopulations in Correlation With Histopathological Effects in Short-Term Inhalation Studies With Biopersistent (Nano)Materials |
VerfasserIn: | Koltermann-Jülly, Johanna Ma-Hock, Lan Gröters, Sibylle Landsiedel, Robert |
Sprache: | Englisch |
Titel: | Toxicologic Pathology |
Bandnummer: | 48 |
Heft: | 3 |
Seiten: | 446-464 |
Verlag/Plattform: | Sage |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | inhalation inflammation particles macrophages M2 polarization state M1 polarization state nanomaterials |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Following inhalation and deposition in the alveolar region at sufficient dose, biopersistent (nano)materials generally provoke pulmonary inflammation. Alveolar macrophages (AMs) are mediators of pulmonary immune responses and were broadly categorized in pro-inflammatory M1 and anti-inflammatory M2 macrophages. This study aimed at identifying AM phenotype as M1 or M2 upon short-term inhalation exposure to different (nano)materials followed by a postexposure period. Phenotyping of AM was retrospectively performed using immunohistochemistry. M1 (CD68þiNOSþ) and M2 (CD68þCD206þ and CD68þArgIþ) AMs were characterized in formalin-fixed, paraffin-embedded lung tissue of rats exposed for 6 hours/day for 5 days to air, 100 mg/m3 nano-TiO2, 25 mg/m3 nano-CeO2, 32 mg/m3 multiwalled carbon nanotubes, or 100 mg/m3 micron-sized quartz. During acute inflammation, relative numbers of M1 AMs were markedly increased, whereas relative numbers of M2 were generally decreased compared to control. Following an exposure-free period, changes in iNOS or CD206 expression correlated with persistence, regression, or progression of inflammation, suggesting a role of M1/M2 AMs in the pathogenesis of pulmonary inflammation. However, no clear correlation of AM subpopulations with qualitatively distinct histopathological findings caused by different (nano)materials was found. A more detailed understanding of the processes underlaying these morphological changes is needed to identify biomarkers for different histopathological outcomes. |
DOI der Erstveröffentlichung: | 10.1177/0192623319896347 |
URL der Erstveröffentlichung: | http://dx.doi.org/10.1177/0192623319896347 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-384956 hdl:20.500.11880/34714 http://dx.doi.org/10.22028/D291-38495 |
ISSN: | 1533-1601 0192-6233 |
Datum des Eintrags: | 9-Dez-2022 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplemental Material |
In Beziehung stehendes Objekt: | https://journals.sagepub.com/doi/suppl/10.1177/0192623319896347/suppl_file/TPX896347_Supplement_Figure_1a-e.tif https://journals.sagepub.com/doi/suppl/10.1177/0192623319896347/suppl_file/TPX896347_Supplement_References.docx |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Pharmazie |
Professur: | NT - Prof. Dr. Claus-Michael Lehr |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.