Please use this identifier to cite or link to this item: doi:10.22028/D291-38495
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Title: Appearance of Alveolar Macrophage Subpopulations in Correlation With Histopathological Effects in Short-Term Inhalation Studies With Biopersistent (Nano)Materials
Author(s): Koltermann-Jülly, Johanna
Ma-Hock, Lan
Gröters, Sibylle
Landsiedel, Robert
Language: English
Title: Toxicologic Pathology
Volume: 48
Issue: 3
Pages: 446-464
Publisher/Platform: Sage
Year of Publication: 2020
Free key words: inhalation
M2 polarization state
M1 polarization state
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Following inhalation and deposition in the alveolar region at sufficient dose, biopersistent (nano)materials generally provoke pulmonary inflammation. Alveolar macrophages (AMs) are mediators of pulmonary immune responses and were broadly categorized in pro-inflammatory M1 and anti-inflammatory M2 macrophages. This study aimed at identifying AM phenotype as M1 or M2 upon short-term inhalation exposure to different (nano)materials followed by a postexposure period. Phenotyping of AM was retrospectively performed using immunohistochemistry. M1 (CD68þiNOSþ) and M2 (CD68þCD206þ and CD68þArgIþ) AMs were characterized in formalin-fixed, paraffin-embedded lung tissue of rats exposed for 6 hours/day for 5 days to air, 100 mg/m3 nano-TiO2, 25 mg/m3 nano-CeO2, 32 mg/m3 multiwalled carbon nanotubes, or 100 mg/m3 micron-sized quartz. During acute inflammation, relative numbers of M1 AMs were markedly increased, whereas relative numbers of M2 were generally decreased compared to control. Following an exposure-free period, changes in iNOS or CD206 expression correlated with persistence, regression, or progression of inflammation, suggesting a role of M1/M2 AMs in the pathogenesis of pulmonary inflammation. However, no clear correlation of AM subpopulations with qualitatively distinct histopathological findings caused by different (nano)materials was found. A more detailed understanding of the processes underlaying these morphological changes is needed to identify biomarkers for different histopathological outcomes.
DOI of the first publication: 10.1177/0192623319896347
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-384956
ISSN: 1533-1601
Date of registration: 9-Dec-2022
Description of the related object: Supplemental Material
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Claus-Michael Lehr
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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