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doi:10.22028/D291-38314
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Titel: | IP3-dependent Ca2+ signals are tightly controlled by Cavβ3, but not by Cavβ1, 2 and 4 |
VerfasserIn: | Belkacemi, Anouar Beck, Andreas Wardas, Barbara ![]() Weissgerber, Petra Flockerzi, Veit ![]() |
Sprache: | Englisch |
In: | |
Titel: | Cell Calcium |
Bandnummer: | 104 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | Voltage-gated Ca2+ channels Cavβ subunits IP3 Ca2+release |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Independent of its function as a subunit of voltage-gated Ca2+ channels, the Cavβ3 subunit desensitizes fibroblasts and pancreatic β-cells to low concentrations of inositol-1,4,5-trisphosphate (IP3). This alters agonist-induced Ca2+ signaling and cellular functions, for example, insulin secretion and wound healing. A total of four Cavβ subunits exist, Cavβ1, Cavβ2, Cavβ3, and Cavβ4. To investigate whether the other Cavβ subunits, like Cavβ3, can desensitize cells to IP3 and thereby modulate Ca2+ signaling, we expressed the cDNAs of Cavβ1, Cavβ2, Cavβ3, and Cavβ4 in COS-7 cells lacking endogenous Cavβ proteins. ATP stimulation of these cells results in the release of Ca2+ from intracellular stores. This receptor-mediated Ca2+ release is significantly decreased by Cavβ3 but not by Cavβ1, Cavβ2, and Cavβ4. Electrophysiological recordings of voltage-dependent Ca2+ currents from fibroblasts show a small Ca2+ current, the amplitude of which is slightly but not significantly smaller in fibroblasts from Cavβ2 gene-deficient animals than in fibroblasts from wild-type animals. Compared with fibroblasts from wild-type animals, Ca2+ release is not significantly increased in Cavβ2-deficient fibroblasts, in contrast to Ca2+ release in Cavβ3-deficient fibroblasts. In summary, our results show that desensitization of cells to low concentrations of IP3 is a specific property of Cavβ3 that is not shared by other Cavβ subunits. |
DOI der Erstveröffentlichung: | 10.1016/j.ceca.2022.102573 |
URL der Erstveröffentlichung: | http://dx.doi.org/10.1016/j.ceca.2022.102573 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-383147 hdl:20.500.11880/34571 http://dx.doi.org/10.22028/D291-38314 |
ISSN: | 0143-4160 |
Datum des Eintrags: | 30-Nov-2022 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Experimentelle und Klinische Pharmakologie und Toxikologie |
Professur: | M - Prof. Dr. Veit Flockerzi |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons