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Titel: Activation of mammalian cytoplasmic dynein in multimotor motility assays
VerfasserIn: Monzon, Gina A.
Scharrel, Lara
Santen, Ludger
Diez, Stefan
Sprache: Englisch
Titel: Journal of Cell Science
Bandnummer: 132 (2019)
Heft: 4
Verlag/Plattform: Company of Biologists Limited
Erscheinungsjahr: 2018
Freie Schlagwörter: Mammalian cytoplasmic dynein
Gliding motility assay
Stochastic modeling
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Long-range intracellular transport is facilitated by motor proteins, such as kinesin-1 and cytoplasmic dynein, moving along microtubules (MTs). These motors often work in teams for the transport of various intracellular cargos. Although transport by multiple kinesin-1 motors has been studied extensively in the past, collective effects of cytoplasmic dynein are less well understood. On the level of single molecules, mammalian cytoplasmic dynein is not active in the absence of dynactin and adaptor proteins. However, when assembled into a team bound to the same cargo, processive motility has been observed. The underlying mechanism of this activation is not known. Here, we found that in MT gliding motility assays the gliding velocity increased with dynein surface density and MT length. Developing a mathematical model based on single-molecule parameters, we were able to simulate the observed behavior. Integral to our model is the usage of an activation term, which describes a mechanical activation of individual dynein motors when being stretched by other motors. We hypothesize that this activation is similar to the activation of single dynein motors by dynactin and adaptor proteins.
DOI der Erstveröffentlichung: 10.1242/jcs.220079
URL der Erstveröffentlichung: http://dx.doi.org/10.1242/jcs.220079
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-382655
hdl:20.500.11880/34529
http://dx.doi.org/10.22028/D291-38265
ISSN: 1477-9137
0021-9533
Datum des Eintrags: 28-Nov-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary information
In Beziehung stehendes Objekt: https://cob.silverchair-cdn.com/cob/content_public/journal/jcs/132/4/10.1242_jcs.220079/5/jcs220079supp.pdf?Expires=1671023629&Signature=Yt6moTR0gnlfWrEvCUujPUDlmoNqjW8QPMEgjNl84Aq8uhXh~cwENSmdrLjl-Wd1m5JK2GUeC6j0J~x4yW5PskK6RAMnKO5xahUcHcDXDPi--fAPYzzvn0e5afhpVhMqNV~PYvjFSLp8pRlT7lJa2BrN7WrUrNmCZyBTlYQHmUlItSAcACW-ZdcRYPa1vMsUJSzwJ0Fr52gG-bFH6~e3I9Ek0xUgWxfGFYkozqjai0rpz57KkdCDcsdzbq2zgWYSen34CwwZh108~WUbcaHjsOx1j6fd448FLVWPEdi3VaADi5DvXwfC9YT6~kfYnXLL3KYG-PQRA-Px9NUa0t5hJg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Prof. Dr. Ludger Santen
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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