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Titel: Glycolytic Shunts Replenish the Calvin-Benson-Bassham Cycle as Anaplerotic Reactions in Cyanobacteria
VerfasserIn: Makowka, Alexander
Nichelmann, Lars
Schulze, Dennis
Spengler, Katharina
Wittmann, Christoph
Forchhammer, Karl
Gutekunst, Kirstin
Sprache: Englisch
Titel: Molecular Plant
Bandnummer: 13
Heft: 3
Seiten: 471-482
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2020
Freie Schlagwörter: central carbohydrate metabolism
Calvin–Benson–Bassham cycle
Entner–Doudoroff pathway
oxidative pentose phosphate pathway
Embden–Meyerhof–Parnas pathway
cyanobacteria
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The recent discovery of the Entner-Doudoroff (ED) pathway as a third glycolytic route beside Embden-Meyerhof-Parnas (EMP) and oxidative pentose phosphate (OPP) pathway in oxygenic photoautotrophs requires a revision of their central carbohydrate metabolism. In this study, unexpectedly, we observed that deletion of the ED pathway alone, and even more pronounced in combination with other glycolytic routes, diminished photoautotrophic growth in continuous light in the cyanobacterium Synechocystis sp. PCC 6803. Furthermore, we found that the ED pathway is required for optimal glycogen catabolism in parallel to an operating Calvin–Benson–Bassham (CBB) cycle. It is counter-intuitive that glycolytic routes, which are a reverse to the CBB cycle and do not provide any additional biosynthetic intermediates, are important under photoautotrophic conditions. However, observations on the ability to reactivate an arrested CBB cycle revealed that they form glycolytic shunts that tap the cellular carbohydrate reservoir to replenish the cycle. Taken together, our results suggest that the classical view of the CBB cycle as an autocatalytic, completely autonomous cycle that exclusively relies on its own enzymes and CO2 fixation to regenerate ribulose-1,5-bisphosphate for Rubisco is an oversimplification. We propose that in common with other known autocatalytic cycles, the CBB cycle likewise relies on anaplerotic reactions to compensate for the depletion of intermediates, particularly in transition states and under fluctuating light conditions that are common in nature.
DOI der Erstveröffentlichung: 10.1016/j.molp.2020.02.002
URL der Erstveröffentlichung: http://dx.doi.org/10.1016/j.molp.2020.02.002
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-382646
hdl:20.500.11880/34528
http://dx.doi.org/10.22028/D291-38264
ISSN: 1674-2052
Datum des Eintrags: 28-Nov-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplemental Information
In Beziehung stehendes Objekt: https://ars.els-cdn.com/content/image/1-s2.0-S1674205220300320-mmc1.pdf
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Christoph Wittmann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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