Please use this identifier to cite or link to this item: doi:10.22028/D291-38141
Title: Bead-based assay for spatiotemporal gene expression control in cell-free transcription-translation systems
Author(s): Finkler, Marc
Ott, Albrecht
Language: English
Title: BioTechniques
Volume: 66
Issue: 1
Pages: 29-33
Publisher/Platform: Future Science
Year of Publication: 2019
Free key words: cell-free expression
Escherichia coli cell extract
transcription control
translation control
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Cell-free gene expression has applications in synthetic biology, biotechnology and biomedicine. In this technique gene expression regulation plays an important role. Transcription factors do not completely suppress expression while other methods for expression control, for example CRISPR/Cas, often require important biochemical modifications. Here we use an all Escherichia coli-based cell-free expression system and present a bead-based method to instantly start and, at a later stage, completely stop gene expression. Magnetic beads coated with DNA of the gene of interest trigger gene expression. The expression stops if we remove the bead-bound DNA as well as transcribed mRNA by hybridization to bead-bound ssDNA. Our method is a simple way to control expression duration very accurately in time and space.
DOI of the first publication: 10.2144/btn-2018-0097
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-381416
ISSN: 1940-9818
Date of registration: 22-Nov-2022
Description of the related object: Supplemental Material
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Albrecht Ott
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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