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Titel: Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox properties
VerfasserIn: Kohlstedt, Michael
Weimer, Anna
Weiland, Fabia
Stolzenberger, Jessica
Selzer, Mirjam
Sanz, Miguel
Kramps, Laurenz
Wittmann, Christoph
Sprache: Englisch
Titel: Metabolic Engineering
Bandnummer: 72
Seiten: 337-352
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2022
Freie Schlagwörter: Pseudomonas putida
Lignin
PET
Catechol
13C metabolic Flux analysis
Transcriptomics
ATP
NADPH
PQQH2
FADH2
Genome reduction
EM42
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Polyethylene terephthalate (PET), the most common synthetic polyester today, is largely produced from fossil resources, contributing to global warming. Consequently, sustainable sources must be developed to meet the increasing demand for this useful polymer. Here, we demonstrate a cascaded value chain that provides green PET from lignin, the world's most underutilized renewable, via fermentative production of cis, cis-muconate (MA) from lignin-based aromatics as a central step. Catechol, industrially the most relevant but apparently also a highly toxic lignin-related aromatic, strongly inhibited MA-producing Pseudomonas putida MA-1. Assessed by 13C metabolic flux analysis, the microbe substantially redirected its carbon core fluxes, resulting in enhanced NADPH supply for stress defense but causing additional ATP costs. The reconstruction of MA production in a genome-reduced P. putida chassis yielded novel producers with superior pathway fluxes and enhanced robustness to catechol and a wide range of other aromatics. Using the advanced producer P. putida MA-10 catechol, MA could be produced in a fed-batch process from catechol (plus glucose as additional growth substrate) up to an attractive titer of 74 g L-1 and a space-time-yield of 1.4 g L-1 h-1. In terms of co-consumed sugar, the further streamlined strain MA-11 achieved the highest yield of 1.4 mol MA (mol glucose)-1, providing a striking economic advantage. Following fermentative production, bio-based MA was purified and used to chemically synthetize the PET monomer terephthalic acid and the comonomer diethylene glycol terephthalic acid through five steps, which finally enabled the first green PET from lignin.
DOI der Erstveröffentlichung: 10.1016/j.ymben.2022.05.001
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/pii/S1096717622000659
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-380249
hdl:20.500.11880/34360
http://dx.doi.org/10.22028/D291-38024
ISSN: 10967176
Datum des Eintrags: 16-Nov-2022
Bezeichnung des in Beziehung stehenden Objekts: Appendix A. Supplementary data
In Beziehung stehendes Objekt: https://ars.els-cdn.com/content/image/1-s2.0-S1096717622000659-mmc1.pdf
https://ars.els-cdn.com/content/image/1-s2.0-S1096717622000659-mmc2.xlsx
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Christoph Wittmann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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