Please use this identifier to cite or link to this item: doi:10.22028/D291-37947
Title: Cannabidiol Exerts a Neuroprotective and Glia-Balancing Effect in the Subacute Phase of Stroke
Author(s): Meyer, Erika
Rieder, Phillip
Gobbo, Davide
Candido, Gabriella
Scheller, Anja
de Oliveira, Rúbia Maria Weffort
Kirchhoff, Frank
Language: English
Title: International Journal of Molecular Sciences
Volume: 23
Issue: 21
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: cannabidiol
in vivo two-photon laserscanning microscopy
Ca2+ signaling
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Pharmacological agents limiting secondary tissue loss and improving functional outcomes after stroke are still limited. Cannabidiol (CBD), the major non-psychoactive component of Cannabis sativa, has been proposed as a neuroprotective agent against experimental cerebral ischemia. The effects of CBD mostly relate to the modulation of neuroinflammation, including glial activation. To investigate the effects of CBD on glial cells after focal ischemia in vivo, we performed time-lapse imaging of microglia and astroglial Ca2+ signaling in the somatosensory cortex in the subacute phase of stroke by in vivo two-photon laser-scanning microscopy using transgenic mice with microglial EGFP expression and astrocyte-specific expression of the genetically encoded Ca2+ sensor GCaMP3. CBD (10 mg/kg, intraperitoneally) prevented ischemia-induced neurological impairment, reducing the neurological deficit score from 2.0 ± 1.2 to 0.8 ± 0.8, and protected against neurodegeneration, as shown by the reduction (more than 70%) in Fluoro-Jade C staining (18.8 ± 7.5 to 5.3 ± 0.3). CBD reduced ischemia-induced microglial activation assessed by changes in soma area and total branch length, and exerted a balancing effect on astroglial Ca2+ signals. Our findings indicate that the neuroprotective effects of CBD may occur in the subacute phase of ischemia, and reinforce its strong anti-inflammatory property. Nevertheless, its mechanism of action on glial cells still requires further studies.
DOI of the first publication: 10.3390/ijms232112886
Link to this record: urn:nbn:de:bsz:291--ds-379479
ISSN: 1422-0067
Date of registration: 11-Nov-2022
Faculty: M - Medizinische Fakultät
Department: M - Physiologie
Professorship: M - Prof. Dr. Frank Kirchhoff
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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