Please use this identifier to cite or link to this item: doi:10.22028/D291-37913
Volltext verfügbar? / Dokumentlieferung
Title: SEC62 and SEC63 Expression in Hepatocellular Carcinoma and Tumor-Surrounding Liver Tissue
Author(s): Casper, Markus
Linxweiler, Maximilian
Linxweiler, Johannes
Zimmermann, Richard
Glanemann, Matthias
Lammert, Frank
Weber, Susanne N.
Language: English
Title: Visceral Medicine
Volume: 37
Issue: 2
Pages: 110–115
Publisher/Platform: Karger
Year of Publication: 2021
Free key words: Chronic liver disease
Endoplasmic reticulum
Gene expression
Intracellular signaling
Liver cancer
Liver tumor
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Introduction: The endoplasmic reticulum transmembrane proteins Sec61, Sec62, and Sec63 are responsible for the intracellular trafficking of precursor proteins and affect intracellular signaling. SEC62 overexpression has been linked to various human cancers. Our aim was to investigate SEC62 and SEC63 expression in hepatocellular carcinoma (HCC) and surrounding liver tissue. Patients and Methods: Primary liver tissue was collected from 11 consecutive patients (70 ± 9 years; 10 men) who underwent HCC resection. In the HCC and the tumor-surrounding liver tissue we investigated SEC62 und SEC63 mRNA expression using quantitative real-time PCR. For Sec62, immunohistochemistry was performed. Results: SEC62and SEC63 total mRNA contents were significantly (p = 0.001) higher in HCCs (CT 22.5 ± 0.4 and 22.6 ± 0.3) when compared to the surrounding tissue (CT 24.6 ± 0.6 and 25.1 ± 0.9). Using the comparative CTmethod, SEC62 and SEC63 expression in HCC was increased 5- and 8.1-fold, respectively, in comparison to surrounding tissue. For Sec62 immunohistochemistry, the mean immunoreactive scores (IRS) were 7.9 ± 2.9 for HCC and 4.8 ± 1.2 for non-tumorous liver (p = 0.027). The mean IRS in HCC were 5.7 ± 3.5 and 8.9 ± 2.3 for patients without (n = 3) and with tumor recurrence (n = 8), respectively. Conclusions: Overexpression of SEC62 and SEC63 is a common feature of HCC. The role of Sec62 as a prognostic marker for tumor recurrence after surgery and its potential role in treatment stratification must be addressed in future studies.
DOI of the first publication: 10.1159/000513293
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-379132
ISSN: 2297-4725
Date of registration: 9-Nov-2022
Description of the related object: Supplementary Material
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Innere Medizin
M - Medizinische Biochemie und Molekularbiologie
Professorship: M - Prof. Dr. Frank Lammert
M - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
There are no files associated with this item.

Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.