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Titel: Towards one-step design of tailored enzymatic nanobiosensors
VerfasserIn: Semenova, Daria
Gernaey, Krist
Morgan, Bruce
Silina, Yuliya
Sprache: Englisch
Titel: The analyst : the analytical journal of the Royal Society of Chemistry
Bandnummer: 145
Heft: 3
Seiten: 1014-1024
Verlag/Plattform: RSC
Erscheinungsjahr: 2020
DDC-Sachgruppe: 540 Chemie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The manufacturing of conventional enzymatic biosensors produced via a layer-by-layer (LbL) approach requires expensive instrumentation, and in most cases involves a complex, resource and time-consuming fabrication process. Moreover, LbL assemblies are prone to mechanical instability that leads to irreversible changes in sensor architecture and morphology resulting in degradation of enzymatic activities and insufficient signal reproducibility. Hence, novel fabrication techniques for the production of enzymatic biosensors that are instrumentally controlled and allow reproducible, simultaneous multi-analyte detection with high specificity, temporal and spatial resolution are greatly required. Herein, we report on the development of a novel, fully instrumentally controlled, one-step synthesis approach for the production of nanoparticle-based enzymatic biosensors. The approach relies on a simultaneous encapsulation of the enzyme (glucose and alcohol oxidases), a fluoropolymer (Nafion) and noble metal nanoparticles via co-deposition from a phosphate multiple electrolyte on top of the sensor surface. Remarkably, electrochemical studies revealed that nanoparticle-based biosensors produced by this novel fabrication approach display a significantly enhanced mechanical stability (more than several orders of magnitude higher) without loss of biological activity or leakage of the enzyme or Nafion, and advanced synthesis reproducibility (40 times higher) in comparison to LbL analogues.
DOI der Erstveröffentlichung: 10.1039/C9AN01745C
URL der Erstveröffentlichung: https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an01745c#!divAbstract
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-304051
hdl:20.500.11880/34209
http://dx.doi.org/10.22028/D291-30405
ISSN: 1364-5528
0003-2654
Datum des Eintrags: 7-Nov-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Bruce Morgan
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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