Please use this identifier to cite or link to this item: doi:10.22028/D291-37740
Title: MicroRNA-30a-5pme : a novel diagnostic and prognostic biomarker for clear cell renal cell carcinoma in tissue and urine samples
Author(s): Outeiro-Pinho, Gonçalo
Barros-Silva, Daniela
Aznar, Elena
Sousa, Ana-Isabel
Vieira-Coimbra, Márcia
Oliveira, Jorge
Gonçalves, Céline S.
Costa, Bruno M.
Junker, Kerstin
Henrique, Rui
Jerónimo, Carmen
Language: English
Title: Journal of Experimental & Clinical Cancer Research
Volume: 39
Issue: 1
Publisher/Platform: Springer Nature
Year of Publication: 2020
Free key words: microRNA
DNA methylation
Clear cell renal cell carcinoma
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background The rising incidence of renal cell carcinomas (RCC) constitutes a significant challenge owing to risk of overtreatment. Because aberrant microRNA (miR) promoter methylation contributes to cancer development, we investigated whether altered miR-30a-5p expression associates with DNA promoter methylation and evaluated the usefulness as clear cell RCC (ccRCC) diagnostic and prognostic markers. Methods Genome-wide methylome and RNA sequencing data from a set of ccRCC and normal tissue samples from The Cancer Genome Atlas (TCGA) database were integrated to identify candidate CpG loci involved in cancer onset. MiR-30a-5p expression and promoter methylation were quantitatively assessed by PCR in a tissue set (Cohort #1) and urine sets (Cohorts #2 and 3) from IPOPorto and Homburg University Hospital. Non-parametric tests were used for comparing continuous variables. MiR-30a-5p promoter methylation (miR-30a-5pme) performance as diagnostic (receiver operator characteristics [ROC] - validity estimates) and prognostic [metastasis-free (MFS) and disease-specific survival (DSS)] biomarker was further validated in urine samples from ccRCC patients by Kaplan Meier curves (with log rank) and both univariable and multivariable analysis. Results Two significant hypermethylated CpG loci in TCGA ccRCC samples, correlating with miR-30a-5p transcriptional downregulation, were disclosed. MiR-30a-5pme in ccRCC tissues was confirmed in an independent patient’s cohort of IPOPorto and associated with shorter time to relapse. In urine samples, miR-30a-5pme levels identified cancer both in testing and validation cohorts, with 83% sensitivity/53% specificity and 63% sensitivity/67% specificity, respectively. Moreover, higher miR-30a-5pme levels independently predicted metastatic dissemination and survival. Conclusion To the best of our knowledge, this is the first study validating the diagnostic and prognostic potential of miR-30a-5pme for ccRCC in urine samples, providing new insights for its clinical usefulness as non-invasive cancer biomarker.
DOI of the first publication: 10.1186/s13046-020-01600-3
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-377409
ISSN: 1756-9966
Date of registration: 27-Oct-2022
Description of the related object: Supplementary information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Urologie und Kinderurologie
Professorship: M - Prof. Dr. Michael Stöckle
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
s13046-020-01600-3.pdf1,96 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons