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doi:10.22028/D291-37644
Titel: | Software for non-parametric image registration of 2-photon imaging data |
VerfasserIn: | Flotho, Philipp Nomura, Shinobu Kuhn, Bernd Strauss, Daniel J. |
Sprache: | Englisch |
Titel: | Journal of Biophotonics |
Bandnummer: | 15 |
Heft: | 8 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | confocal microscopy image registration ImageJ/FIJI plugin MATLAB toolbox movement correction optical flow optical imaging two-photon microscopy |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Functional 2-photon microscopy is a key technology for imaging neuronal activity. The recorded image sequences, however, can contain non-rigid movement artifacts which requires high-accuracy movement correction. Variational optical flow (OF) estimation is a group of methods for motion analysis with established performance in many computer vision areas. However, it has yet to be adapted to the statistics of 2-photon neuroimaging data. In this work, we present the motion compensation method Flow-Registration that outperforms previous alignment tools and allows to align and reconstruct even low signal-to-noise ratio 2-photon imaging data and is able to compensate high-divergence displacements during local drug injections. The method is based on statistics of such data and integrates previous advances in variational OF estimation. Our method is available as an easy-to-use ImageJ/FIJI plugin as well as a MATLAB toolbox with modular, object oriented file IO, native multi-channel support and compatibility with existing 2-photon. |
DOI der Erstveröffentlichung: | 10.1002/jbio.202100330 |
URL der Erstveröffentlichung: | https://onlinelibrary.wiley.com/doi/10.1002/jbio.202100330 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-376445 hdl:20.500.11880/34059 http://dx.doi.org/10.22028/D291-37644 |
ISSN: | 1864-0648 1864-063X |
Datum des Eintrags: | 18-Okt-2022 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fjbio.202100330&file=jbio202100330-sup-0001-AppendixS1.zip |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Neurochirurgie |
Professur: | M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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Journal of Biophotonics - 2022 - Flotho - Software for non‐parametric image registration of 2‐photon imaging data.pdf | 10,17 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons