Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen: doi:10.22028/D291-37620
Titel: MicroRNA-targeting in male infertility: Sperm microRNA-19a/b-3p and its spermatogenesis related transcripts content in men with oligoasthenozoospermia
VerfasserIn: Abu-Halima, Masood
Becker, Lea Simone
Ayesh, Basim M.
Meese, Eckart
Sprache: Englisch
Titel: Frontiers in Cell and Developmental Biology
Bandnummer: 10
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2022
Freie Schlagwörter: male subfertility
oligoasthenozoospermia
expression analysis
miR-19a-3p
miR19b-3p
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Objective: To elucidate and validate the potential regulatory function of miR-19a/b-3p and its spermatogenesis-related transcripts content in sperm samples collected from men with oligoasthenozoospermia. Methods: Men presenting at an infertility clinic were enrolled. MicroRNA (miRNA) and target genes evaluation were carried out using in silico prediction analysis, Reverse transcription-quantitative PCR (RT-qPCR) validation, and Western blot confirmation. Results: The expression levels of miRNA-19a/b-3p were significantly upregulated and 51 target genes were significantly down-regulated in oligoasthenozoospermic men compared with age-matched normozoospermic men as determined by RT-qPCR. Correlation analysis highlighted that sperm count, motility, and morphology were negatively correlated with miRNA-19a/b-3p and positively correlated with the lower expression level of 51 significantly identified target genes. Furthermore, an inverse correlation between higher expression levels of miRNA-19a/b-3p and lower expression levels of 51 target genes was observed. Consistent with the results of the RT-qPCR, reduced expression levels of STK33 and DNAI1 protein levels were identified in an independent cohort of sperm samples collected from men with oligoasthenozoospermia. Conclusion: Findings suggest that the higher expression of miRNA-19a/b3p or the lower expression of target genes are associated with oligoasthenozoospermia and male infertility, probably through influencing basic semen parameters. This study lay the groundwork for future studies focused on investigating therapies for male infertility.
DOI der Erstveröffentlichung: 10.3389/fcell.2022.973849
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fcell.2022.973849/full
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-376206
hdl:20.500.11880/34038
http://dx.doi.org/10.22028/D291-37620
ISSN: 2296-634X
Datum des Eintrags: 17-Okt-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_tables_1_docx/octet-stream/Table%201.DOCX/1/973849
https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_images_1_tif/octet-stream/Image%201.TIF/1/973849
https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_images_2_tiff/octet-stream/Image%202.TIFF/1/973849
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Humangenetik
Professur: M - Prof. Dr. Eckhart Meese
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Dateien zu diesem Datensatz:
Datei Beschreibung GrößeFormat 
fcell-10-973849.pdf1,55 MBAdobe PDFÖffnen/Anzeigen


Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons