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doi:10.22028/D291-37620
Titel: | MicroRNA-targeting in male infertility: Sperm microRNA-19a/b-3p and its spermatogenesis related transcripts content in men with oligoasthenozoospermia |
VerfasserIn: | Abu-Halima, Masood Becker, Lea Simone Ayesh, Basim M. Meese, Eckart |
Sprache: | Englisch |
Titel: | Frontiers in Cell and Developmental Biology |
Bandnummer: | 10 |
Verlag/Plattform: | Frontiers |
Erscheinungsjahr: | 2022 |
Freie Schlagwörter: | male subfertility oligoasthenozoospermia expression analysis miR-19a-3p miR19b-3p |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Objective: To elucidate and validate the potential regulatory function of miR-19a/b-3p and its spermatogenesis-related transcripts content in sperm samples collected from men with oligoasthenozoospermia. Methods: Men presenting at an infertility clinic were enrolled. MicroRNA (miRNA) and target genes evaluation were carried out using in silico prediction analysis, Reverse transcription-quantitative PCR (RT-qPCR) validation, and Western blot confirmation. Results: The expression levels of miRNA-19a/b-3p were significantly upregulated and 51 target genes were significantly down-regulated in oligoasthenozoospermic men compared with age-matched normozoospermic men as determined by RT-qPCR. Correlation analysis highlighted that sperm count, motility, and morphology were negatively correlated with miRNA-19a/b-3p and positively correlated with the lower expression level of 51 significantly identified target genes. Furthermore, an inverse correlation between higher expression levels of miRNA-19a/b-3p and lower expression levels of 51 target genes was observed. Consistent with the results of the RT-qPCR, reduced expression levels of STK33 and DNAI1 protein levels were identified in an independent cohort of sperm samples collected from men with oligoasthenozoospermia. Conclusion: Findings suggest that the higher expression of miRNA-19a/b3p or the lower expression of target genes are associated with oligoasthenozoospermia and male infertility, probably through influencing basic semen parameters. This study lay the groundwork for future studies focused on investigating therapies for male infertility. |
DOI der Erstveröffentlichung: | 10.3389/fcell.2022.973849 |
URL der Erstveröffentlichung: | https://www.frontiersin.org/articles/10.3389/fcell.2022.973849/full |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-376206 hdl:20.500.11880/34038 http://dx.doi.org/10.22028/D291-37620 |
ISSN: | 2296-634X |
Datum des Eintrags: | 17-Okt-2022 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Material |
In Beziehung stehendes Objekt: | https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_tables_1_docx/octet-stream/Table%201.DOCX/1/973849 https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_images_1_tif/octet-stream/Image%201.TIF/1/973849 https://www.frontiersin.org/articles/file/downloadfile/973849_supplementary-materials_images_2_tiff/octet-stream/Image%202.TIFF/1/973849 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Humangenetik |
Professur: | M - Prof. Dr. Eckhart Meese |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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fcell-10-973849.pdf | 1,55 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons