Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-37617
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Titel: | Search and Capture Efficiency of Dynamic Microtubules for Centrosome Relocation during IS Formation |
VerfasserIn: | Sarkar, Apurba Rieger, Heiko ![]() Paul, Raja |
Sprache: | Englisch |
In: | |
Titel: | Biophysical Journal |
Bandnummer: | 116 |
Heft: | 11 |
Seiten: | 2079–2091 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2019 |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Upon contact with antigen-presenting cells, cytotoxic T lymphocytes (T cells) establish a highly organized contact zone denoted as the immunological synapse (IS). The formation of the IS implies relocation of the microtubule organizing center (MTOC) toward the contact zone, which necessitates a proper connection between the MTOC and the IS via dynamic microtubules (MTs). The efficiency of the MTs finding the IS within the relevant timescale is, however, still illusive. We investigate how MTs search the three-dimensional constrained cellular volume for the IS and bind upon encounter to dynein anchored at the IS cortex. The search efficiency is estimated by calculating the time required for the MTs to reach the dynein-enriched region of the IS. In this study, we develop simple mathematical and numerical models incorporating relevant components of a cell and propose an optimal search strategy. Using the mathematical model, we have quantified the average search time for a wide range of model parameters and proposed an optimized set of values leading to the minimal capture time. Our results show that search times are minimal when the IS formed at the nearest or at the farthest sites on the cell surface with respect to the perinuclear MTOC. The search time increases monotonically away from these two specific sites and is maximal at an intermediate position near the equator of the cell. We observed that search time strongly depends on the number of searching MTs and distance of the MTOC from the nuclear surface. |
DOI der Erstveröffentlichung: | 10.1016/j.bpj.2019.04.008 |
URL der Erstveröffentlichung: | https://www.sciencedirect.com/science/article/pii/S000634951930308X |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-376171 hdl:20.500.11880/34031 http://dx.doi.org/10.22028/D291-37617 |
ISSN: | 0006-3495 |
Datum des Eintrags: | 14-Okt-2022 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Material |
In Beziehung stehendes Objekt: | https://ars.els-cdn.com/content/image/1-s2.0-S000634951930308X-mmc1.pdf https://ars.els-cdn.com/content/image/1-s2.0-S000634951930308X-mmc2.pdf |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Physik |
Professur: | NT - Prof. Dr. Heiko Rieger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.