Please use this identifier to cite or link to this item: doi:10.22028/D291-37460
Title: New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme
Author(s): Paulus, Constanze
Rebets, Yuriy
Zapp, Josef
Rückert, Christian
Kalinowski, Jörn
Luzhetskyy, Andriy
Language: English
Title: Frontiers in Microbiology
Volume: 9
Publisher/Platform: Frontiers
Year of Publication: 2018
Free key words: Streptomyces
secondary metabolites
NRPS-trans-AT-polyketide synthase
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: The environment of Lake Baikal is a well-known source of microbial diversity. The strain Streptomyces sp. IB2014/011-12, isolated from samples collected at Lake Baikal, was found to exhibit potent activity against Gram-positive bacteria. Here, we report isolation and characterization of linear polyketide alpiniamide A (1) and its new derivatives B–D (2–5). The structures of alpiniamides A–D were established and their relative configuration was determined by combination of partial Murata’s method and ROESY experiment. The absolute configuration of alpiniamide A was established through Mosher’s method. The gene cluster, responsible for the biosynthesis of alpiniamides (alp) has been identified by genome mining and gene deletion experiments. The successful expression of the cloned alp gene cluster in a heterologous host supports these findings. Analysis of the architecture of the alp gene cluster and the feeding of labeled precursors elucidated the alpiniamide biosynthetic pathway. The biosynthesis of alpiniamides is an example of a rather simple polyketide assembly line generating unusual chemical diversity through the combination of domain/module skipping and double bond migration events.
DOI of the first publication: 10.3389/fmicb.2018.01959
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-374604
ISSN: 1664-302X
Date of registration: 30-Sep-2022
Description of the related object: Supplementary Material
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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