Please use this identifier to cite or link to this item: doi:10.22028/D291-37455
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Title: Transgenic expression of the RNA binding protein IMP2 stabilizes miRNA targets in murine microsteatosis
Author(s): Dehghani Amirabad, Azim
Ramasamy, Pathmanaban
Wierz, Marina
Nordström, Karl
Kessler, Sonja M.
Schulz, Marcel H.
Simon, Martin
Language: English
Title: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Volume: 1864
Issue: 10
Pages: 3099-3108
Publisher/Platform: Elsevier
Year of Publication: 2018
Free key words: IGF2
Murine steatosis
DDC notations: 500 Science
610 Medicine and health
Publikation type: Journal Article
Abstract: Adult expression of IMP2 is often associated with several types of disease and cancer. The RNA binding protein IMP2 binds and stabilizes the IGF2 mRNA as well as hundreds of other transcripts during development. To gain insight into the molecular action of IMP2 and its contribution to disease in context of adult cellular metabolism, we analyze transgenic overexpression of IMP2 in mouse livers, which has been shown to induce a steatosis-like phenotype and enhanced risk to develop hepatocellular carcinoma (HCC). Our data show up-regulation of several HCC marker genes and miRNAs (miR438-3p and miR151-5p). To characterize the impact of miRNAs to their targets, integrative analysis of transcriptome-and miRNAome-dynamics in combination with IMP2 target prediction was carried out. Our analyses show that targets of expressed miRNAs become accumulated in the case that these transcripts have positive IMP2 binding prediction. Therefore, our data indicates that overexpression of IMP2 alters the regulatory capacity of many miRNAs and we conclude that IMP2 competes with miRNAs for binding sites on thousands of transcripts. As a result, our data implicates that overexpression of IMP2 has distinct effects to the regulatory capacity of miRNAs with yet unknown consequences for translational efficiency.
DOI of the first publication: 10.1016/j.bbadis.2018.05.024
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-374555
ISSN: 0925-4439
Date of registration: 29-Sep-2022
Description of the related object: Supplementary data
Related object:
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Experimentelle und Klinische Pharmakologie und Toxikologie
NT - Biowissenschaften
NT - Pharmazie
Professorship: M - Keiner Professur zugeordnet
NT - Prof. Dr. Alexandra K. Kiemer
NT - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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