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Titel: Manganese-Induced Toxicity in C. elegans : What Can We Learn from the Transcriptome?
VerfasserIn: Nicolai, Merle M.
Pirritano, Marcello
Gasparoni, Gilles
Aschner, Michael
Simon, Martin
Bornhorst, Julia
Sprache: Englisch
Titel: International Journal of Molecular Sciences
Bandnummer: 23
Heft: 18
Verlag/Plattform: MDPI
Erscheinungsjahr: 2022
Freie Schlagwörter: transcriptome analysis
manganese
Caenorhabditis elegans
metal response
oxidative stress
stress response
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Manganese (Mn) is an essential ubiquitous transition metal and, when occupationally or environmentally overexposed, a well-known risk factor for several neurological pathologies. However, the molecular mechanisms underlying Mn-induced neurotoxicity are largely unknown. In this study, addressing RNA-Seq analysis, bioavailability and survival assays, key pathways of transcriptional responses to Mn overexposure were investigated in the model organism Caenorhabditis elegans (C. elegans), providing insights into the Mn-induced cellular stress and damage response. Comparative transcriptome analyses identified a large number of differentially expressed genes (DEGs) in nematodes exposed to MnCl2 , and functional annotation suggested oxidative nucleotide damage, unfolded protein response and innate immunity as major damage response pathways. Additionally, a time-dependent increase in the transcriptional response after MnCl2 exposure was identified by means of increased numbers of DEGs, indicating a time-dependent response and activation of the stress responses in Mn neurotoxicity. The data provided here represent a powerful transcriptomic resource in the field of Mn toxicity, and therefore, this study provides a useful basis for further planning of targeted mechanistic studies of Mn-induced neurotoxicity that are urgently needed in the face of increasing industrially caused environmental pollution with Mn.
DOI der Erstveröffentlichung: 10.3390/ijms231810748
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-373410
hdl:20.500.11880/33828
http://dx.doi.org/10.22028/D291-37341
ISSN: 1422-0067
Datum des Eintrags: 26-Sep-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/article/10.3390/ijms231810748/s1
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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