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Titel: Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
VerfasserIn: Kolberg, Adrianna
Wenzel, Christiane
Hugel, Thorsten
Gallei, Markus
Balzer, Bizan N.
Sprache: Englisch
Titel: Journal of visualized experiments : JoVE
Heft: 157
Verlag/Plattform: JoVE
Erscheinungsjahr: 2020
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Atomic force microscopy (AFM)-based single molecule force spectroscopy is an ideal tool for investigating the interactions between a single polymer and surfaces. For a true single molecule experiment, covalent attachment of the probe molecule is essential because only then can hundreds of force-extension traces with one and the same single molecule be obtained. Many traces are in turn necessary to prove that a single molecule alone is probed. Additionally, passivation is crucial for preventing unwanted interactions between the single probe molecule and the AFM cantilever tip as well as between the AFM cantilever tip and the underlying surface. The functionalization protocol presented here is reliable and can easily be applied to a variety of polymers. Characteristic single molecule events (i.e., stretches and plateaus) are detected in the force-extension traces. From these events, physical parameters such as stretching force, desorption force and desorption length can be obtained. This is particularly important for the precise investigation of stimuli-responsive systems at the single molecule level. As exemplary systems poly(ethylene glycol) (PEG), poly(N-isopropylacrylamide) (PNiPAM) and polystyrene (PS) are stretched and desorbed from SiOx (for PEG and PNiPAM) and from hydrophobic self-assembled monolayer surfaces (for PS) in aqueous environment.
DOI der Erstveröffentlichung: 10.3791/60934
URL der Erstveröffentlichung: https://www.jove.com/de/t/60934/covalent-attachment-single-molecules-for-afm-based-force
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-371374
hdl:20.500.11880/33704
http://dx.doi.org/10.22028/D291-37137
ISSN: 1940-087X
Datum des Eintrags: 1-Sep-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Markus Gallei
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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