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Titel: The Impact of the Functional Layer Composition of Glucose Test-Strips on the Stability of Electrochemical Response
VerfasserIn: Zolotukhina, Ekaterina V.
Gerasimova, Ekaterina V.
Sorokin, Vladislav V.
Levchenko, Maria G.
Freiman, Alisa S.
Silina, Yuliya E.
Sprache: Englisch
Titel: Chemosensors
Bandnummer: 10
Heft: 8
Verlag/Plattform: MDPI
Erscheinungsjahr: 2022
Freie Schlagwörter: glucose test strips
alginate
RedOx mediator
stability
functional layer
analytical performance
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Herein, the impact of the chemical stability of RedOx mediator ferricyanide, K3 [Fe(CN)6 ] (FC), a type of buffer solution used for bioreceptor preparation, gel composition (carboxymethylcellulose, CMC, Aerosile, AS, and alginate, ALG) on the long term stability of glucose test-strips and their analytical performance was examined. By simple addition of ALG to the functional gel aiming to improve its viscosity, we managed to enhance the sensitivity of conventional CMC-containing amperometric glucose test-strips from 3.3 µA/mM to 3.9 µA/mM and extend their shelf life from 8 months to 1.7 years. Moreover, during the course of investigations, it was revealed that the activity of enzyme in dependence with the used buffer did not linearly correlate with its activity in a dried functional layer, and the entire long-term electrochemical signal of glucose test-strips was determined by RedOx mediator FC chemical stability. The most stable and sensitive test-strips were obtained by the screen-printing approach from a gel containing 24 mg/mL GOx prepared in citrate buffer with pH 6, 200 mg/mL of FC and 10 mg/mL of CMC supplemented with 25 mg/mL of ALG.
DOI der Erstveröffentlichung: 10.3390/chemosensors10080298
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-370837
hdl:20.500.11880/33664
http://dx.doi.org/10.22028/D291-37083
ISSN: 2227-9040
Datum des Eintrags: 26-Aug-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/article/10.3390/chemosensors10080298/s1
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Bruce Morgan
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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