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Titel: Micro-rheological properties of lung homogenates correlate with infection severity in a mouse model of Pseudomonas aeruginosa lung infection
VerfasserIn: Murgia, Xabier
Kany, Andreas M.
Herr, Christian
Ho, Duy-Khiet
De Rossi, Chiara
Bals, Robert
Lehr, Claus-Michael
Hirsch, Anna K. H.
Hartmann, Rolf W.
Empting, Martin
Röhrig, Teresa
Sprache: Englisch
Titel: Scientific Reports
Bandnummer: 10
Heft: 1
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2020
Freie Schlagwörter: Biomarkers
Drug development
Infection
DDC-Sachgruppe: 500 Naturwissenschaften
610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Lung infections caused by Pseudomonas aeruginosa pose a serious threat to patients suffering from, among others, cystic fibrosis, chronic obstructive pulmonary disease, or bronchiectasis, often leading to life-threatening complications. The establishment of a chronic infection is substantially related to communication between bacteria via quorum-sensing networks. In this study, we aimed to assess the role of quorum-sensing signaling molecules of the Pseudomonas quinolone signal (PQS) and to investigate the viscoelastic properties of lung tissue homogenates of PA-infected mice in a prolonged acute murine infection model. Therefore, a murine infection model was successfully established via intra-tracheal infection with alginate-supplemented Pseudomonas aeruginosa NH57388A. Rheological properties of lung homogenates were analyzed with multiple particle tracking (MPT) and quorum-sensing molecules were quantified with LC–MS/MS. Statistical analysis of bacterial load and quorum-sensing molecules showed a strong correlation between these biomarkers in infected lungs. This was accompanied by noticeable changes in the consistency of lung homogenates with increasing infection severity. Furthermore, viscoelastic properties of the lung homogenates strongly correlated with bacterial load and quorum sensing molecules. Considering the strong correlation between the viscoelasticity of lung homogenates and the aforementioned biomarkers, the viscoelastic properties of infected lungs might serve as reliable new biomarker for the evaluation of the severity of P. aeruginosa infections in murine models.
DOI der Erstveröffentlichung: 10.1038/s41598-020-73459-5
URL der Erstveröffentlichung: https://www.nature.com/articles/s41598-020-73459-5
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-367736
hdl:20.500.11880/33413
http://dx.doi.org/10.22028/D291-36773
ISSN: 2045-2322
Datum des Eintrags: 12-Jul-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary information
In Beziehung stehendes Objekt: https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-020-73459-5/MediaObjects/41598_2020_73459_MOESM1_ESM.pdf
https://www.nature.com/articles/s41598-020-73459-5#Sec12
Fakultät: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: M - Innere Medizin
NT - Pharmazie
Professur: M - Prof. Dr. Robert Bals
NT - Prof. Dr. Rolf W. Hartmann
NT - Prof. Dr. Anna Hirsch
NT - Prof. Dr. Claus-Michael Lehr
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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