Please use this identifier to cite or link to this item: doi:10.22028/D291-36553
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Title: PCR-based verification of positive rapid diagnostic tests for intestinal protozoa infections with variable test band intensity
Author(s): Becker, Sören
Müller, Ivan
Mertens, Pascal
Herrmann, Mathias
Zondie, Leyli
Beyleveld, Lindsey
Gerber, Markus
du Randt, Rosa
Pühse, Uwe
Walter, Cheryl
Utzinger, Jürg
Language: English
Title: Acta Tropica
Volume: 174
Pages: 49-55
Publisher/Platform: Elsevier
Year of Publication: 2017
Free key words: Cryptosporidium spp.
Giardia intestinalis
Intestinal protozoa
Multiplex PCR
Rapid diagnostic test
South Africa
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Stool-based rapid diagnostic tests (RDTs) for pathogenic intestinal protozoa (e.g. Cryptosporidium spp. and Giardia intestinalis) allow for prompt diagnosis and treatment in resource-constrained settings. Such RDTs can improve individual patient management and facilitate population-based screening programmes in areas without microbiological laboratories for confirmatory testing. However, RDTs are difficult to interpret in case of ‘trace’ results with faint test band intensities and little is known about whether such ambiguous results might indicate ‘true’ infections. In a longitudinal study conducted in poor neighbourhoods of Port Elizabeth, South Africa, a total of 1428 stool samples from two cohorts of schoolchildren were examined on the spot for Cryptosporidium spp. and G. intestinalis using an RDT (Crypto/Giardia DuoStrip; Coris BioConcept). Overall, 121 samples were positive for G. intestinalis and the RDT suggested presence of cryptosporidiosis in 22 samples. After a storage period of 9–10 months in cohort 1 and 2–3 months in cohort 2, samples were subjected to multiplex PCR (BD Max™ Enteric Parasite Panel, Becton Dickinson). Ninety-three percent (112/121) of RDT-positive samples for G. intestinalis were confirmed by PCR, with a correlation between RDT test band intensity and quantitative pathogen load present in the sample. For Cryptosporidium spp., all positive RDTs had faintly visible lines and these were negative on PCR. The performance of the BD Max™ PCR was nearly identical in both cohorts, despite the prolonged storage at disrupted cold chain conditions in cohort 1. The Crypto/Giardia DuoStrip warrants further validation in communities with a high incidence of diarrhoea.
DOI of the first publication: 10.1016/j.actatropica.2017.06.012
URL of the first publication: https://www.sciencedirect.com/science/article/pii/S0001706X17304151
Link to this record: urn:nbn:de:bsz:291--ds-365538
hdl:20.500.11880/33205
http://dx.doi.org/10.22028/D291-36553
ISSN: 0001-706X
Date of registration: 22-Jun-2022
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Sören Becker
Collections:Die Universitätsbibliographie

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