Please use this identifier to cite or link to this item: doi:10.22028/D291-36540
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Title: Diagnostic comparison of Baermann funnel, Koga agar plate culture and polymerase chain reaction for detection of human Strongyloides stercoralis infection in Maluku, Indonesia
Author(s): Kristanti, Handriani
Meyanti, Fransiska
Wijayanti, Mahardika Agus
Mahendradhata, Yodi
Polman, Katja
Chappuis, François
Utzinger, Jürg
Becker, Sören L.
Murhandarwati, E. Elsa Herdiana
Language: English
Title: Parasitology Research
Volume: 117
Issue: 10
Pages: 3229–3235
Publisher/Platform: Springer Nature
Year of Publication: 2018
Free key words: Baermann funnel
ITS2 region
Koga agar plate culture
Polymerase chain reaction
Soil-transmitted helminthiasis
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Human infection with the nematode Strongyloides stercoralis, which may have a life-threatening course, primarily occurs in tropical settings. Epidemiological data on the occurrence of strongyloidiasis are scarce, and microscopic stool-based detection methods are insensitive. Polymerase chain reaction (PCR) assays have been developed, yet conflicting results have been reported. Our goal was to determine whether there was diagnostic agreement between an inhouse PCR and two microscopic techniques, the Baermann funnel (BM) and the Koga agar plate culture (KAP) for the detection of S. stercoralis in stool samples. Eighty ethanol-fixed stool samples stemming from a cross-sectional survey in Maluku, Indonesia, were purposefully selected for PCR analysis. The final sample size comprised four groups, each with 20 samples: group 1, positive for S. stercoralis on both BM and KAP; group 2, positive only by BM; group 3, positive only by KAP; and group 4, negative on both BM and KAP. A Strongyloides-specific PCR targeting the internal transcribed spacer 2 (ITS2) region was carried out in an Indonesian reference laboratory. The overall agreement between PCR and microscopy was 61% (49/80 samples), being highest in group 1 (15/20, 75%) and lowest in group 3 (9/20, 45%). PCR revealed eight additional S. stercoralis infections in group 4. Future studies should elucidate the ‘true’ infection status of samples that are negative by PCR, but positive upon microscopy. Taken together, there is a lack of agreement between microscopy and PCR results for the diagnosis of human S. stercoralis infection in Indonesia. (identifier: NCT02105714)
DOI of the first publication: 10.1007/s00436-018-6021-5
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-365409
ISSN: 1432-1955
Date of registration: 22-Jun-2022
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Sören Becker
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