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Titel: Cinobufacini Injection Inhibits the Proliferation of Triple-Negative Breast Cancer Through the Pin1-TAZ Signaling Pathway
VerfasserIn: Kong, Lu
Liu, Xu
Yu, Bing
Yuan, Ye
Zhao, Qianru
Chen, Yuru
Qu, Bin
Du, Xue
Tian, Xiaoxuan
Shao, Rui
Wang, Yu
Sprache: Englisch
Titel: Frontiers in pharmacology
Bandnummer: 13
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2022
Freie Schlagwörter: cinobufacini injection
triple-negative breast cancer
TAZ
Pin1
proliferation
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer (BC), which is characterized by the total absence of human epidermal growth factor receptor 2 (HER2), progesterone receptor (PR), and estrogen receptor (ER) expression. Cinobufacini injection (CI) is the aqueous extract from the dry skin of Bufo gargarizans, which is broadly used for the treatment of malignant tumors. However, the potential mechanism of CI against TNBC has not been fully revealed. In this study, we found that CI inhibited the proliferation of MDA-MB-231 and 4T1 cells in a time- and dose-dependent manner. RNA-seq data showed that downregulated and upregulated genes were mainly enriched in biological processes related to tumor cell proliferation, including cell cycle arrest and regulation of apoptosis signaling pathways. Indeed, after CI treatment, the protein level of CDK1 and Bcl-2/Bax decreased, indicating that CI induced the cell cycle of MDA-MB-231 arrest in the G2/M phase and increased the rate of apoptosis. Meanwhile, CI significantly inhibited the growth of tumor in vivo, and RNA-seq data showed that the TAZ signaling pathway played a vital role after CI treatment. Both immunohistochemistry and Western blot analysis confirmed the downregulation of Pin1 and TAZ, caused by CI treatment. Furthermore, the bioinformatics analysis indicated that Pin1 and TAZ were indeed elevated in TNBC patients, with poor staging, classification, and patient survival rate. In conclusion, CI effectively inhibited the proliferation of TNBC in vitro and in vivo and induced their apoptosis and cycle arrest through the Pin1-TAZ pathway.
DOI der Erstveröffentlichung: 10.3389/fphar.2022.797873
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fphar.2022.797873/full
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-362322
hdl:20.500.11880/33118
http://dx.doi.org/10.22028/D291-36232
ISSN: 1663-9812
Datum des Eintrags: 15-Jun-2022
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Biophysik
Professur: M - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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