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|Title:||The ischemic time window of ectopic endometrial tissue crucially determines its ability to develop into endometriotic lesions|
Huwer, Sarah I.
Menger, Michael D.
Laschke, Matthias W.
|Year of Publication:||2022|
|Free key words:||Experimental models of disease|
|DDC notations:||610 Medicine and health|
|Publikation type:||Journal Article|
|Abstract:||Endometriosis develop from shed endometrial fragments via retrograde menstruation. This afects the survival, proliferation and vascularization of the tissue and its fnal ability to form endometriotic lesions. Within this study, uterine tissue samples from donor mice were precultivated for 24 h or 72 h to simulate avascular periods. Their morphology, microvessel density, apoptotic activity and expression of angiogenesis-related proteins were analyzed in vitro. The formation of endometriotic lesions in vivo was assessed after transplantation of precultivated uterine tissue samples to the abdominal wall and dorsal skinfold chambers by means of high-resolution ultrasound, intravital fuorescence microscopy, histology and immunohistochemistry. In vitro, 72-h-precultivated uterine tissue samples exhibit extensive areas of tissue necrosis and high numbers of apoptotic cells as well as a signifcantly reduced cell and microvessel density. These samples failed to develop into endometriotic lesions. In contrast, the 24-h-precultivated samples showed, that their early vascularization and growth in vivo was improved when compared to controls. This indicates that avascular periods have a strong impact on the survival of ectopic endometrial tissue and the chance for the development of endometriosis.|
|DOI of the first publication:||10.1038/s41598-022-09577-z|
|URL of the first publication:||https://www.nature.com/articles/s41598-022-09577-z|
|Link to this record:||urn:nbn:de:bsz:291--ds-363949|
|Date of registration:||8-Jun-2022|
|Description of the related object:||Supplementary Information|
|Faculty:||M - Medizinische Fakultät|
|Department:||M - Chirurgie|
|Professorship:||M - Prof. Dr. Michael D. Menger|
|Collections:||SciDok - Der Wissenschaftsserver der Universität des Saarlandes|
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