Please use this identifier to cite or link to this item: doi:10.22028/D291-36394
Title: The ischemic time window of ectopic endometrial tissue crucially determines its ability to develop into endometriotic lesions
Author(s): Rudzitis-Auth, Jeannette
Huwer, Sarah I.
Scheuer, Claudia
Menger, Michael D.
Laschke, Matthias W.
Language: English
Title: Scientific Reports
Volume: 12
Issue: 1
Publisher/Platform: Springer Nature
Year of Publication: 2022
Free key words: Experimental models of disease
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Endometriosis develop from shed endometrial fragments via retrograde menstruation. This afects the survival, proliferation and vascularization of the tissue and its fnal ability to form endometriotic lesions. Within this study, uterine tissue samples from donor mice were precultivated for 24 h or 72 h to simulate avascular periods. Their morphology, microvessel density, apoptotic activity and expression of angiogenesis-related proteins were analyzed in vitro. The formation of endometriotic lesions in vivo was assessed after transplantation of precultivated uterine tissue samples to the abdominal wall and dorsal skinfold chambers by means of high-resolution ultrasound, intravital fuorescence microscopy, histology and immunohistochemistry. In vitro, 72-h-precultivated uterine tissue samples exhibit extensive areas of tissue necrosis and high numbers of apoptotic cells as well as a signifcantly reduced cell and microvessel density. These samples failed to develop into endometriotic lesions. In contrast, the 24-h-precultivated samples showed, that their early vascularization and growth in vivo was improved when compared to controls. This indicates that avascular periods have a strong impact on the survival of ectopic endometrial tissue and the chance for the development of endometriosis.
DOI of the first publication: 10.1038/s41598-022-09577-z
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-363949
ISSN: 2045-2322
Date of registration: 8-Jun-2022
Description of the related object: Supplementary Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
s41598-022-09577-z.pdf13,73 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons