Please use this identifier to cite or link to this item: doi:10.22028/D291-36361
Title: Intravenous propofol, ketamine (ketofol) and rocuronium after sevoflurane induction provides long lasting anesthesia in ventilated rats
Author(s): Kiefer, Daniel
Müller-Wirtz, Lukas M.
Maurer, Felix
Hüppe, Tobias
Mathes, Alexander M.
Volk, Thomas
Kreuer, Sascha
Fink, Tobias
Language: English
Title: Experimental Animals
Volume: 71
Issue: 2
Pages: 231-239
Publisher/Platform: Japanese Association for Laboratory Animal Science
Year of Publication: 2021
Free key words: anesthetics plasma concentration
rat anesthesia techniques
rat inhalation anesthesia
rat long time anesthesia
rat ventilation
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Rats are commonly used animals for laboratory experiments and many experiments require general anesthesia. However, the lack of published and reproducible intravenous anesthesia protocols for rats results in unnecessary animal use to establish new anesthesia techniques across institutions. We therefore developed an anesthesia protocol with propofol, ketamine, and rocuronium for mechanically ventilated rats, and evaluated vital parameters and plasma concentrations. 15 male Sprague-Dawley rats underwent inhalation induction with sevoflurane and tracheal, venous and arterial cannulation. After established venous access, sevoflurane was substituted by propofol and ketamine (ketofol). Rocuronium was added under mechanical ventilation for 7 h. Drug dosages were stepwise reduced to prevent accumulation. All animals survived the observation period and showed adequate depth of anesthesia. Mean arterial pressure and heart rate remained within normal ranges. Median propofol plasma concentrations remained stable: 1, 4, 7 h: 2.0 (interquartile range (IQR): 1.8–2.2), 2.1 (1.8–2.2), 1.8 (1.6–2.1) µg/ml, whereas median ketamine concentrations slightly differed after 7 h compared to 1 h: 1, 4, 7 h: 3.7 (IQR: 3.5–4.5), 3.8 (3.3–4.1), 3.8 (3.0–4.1) µg/ml. Median rocuronium plasma concentrations were lower after 4 and 7 h compared to 1 h: 1, 4, 7 h: 3.9 (IQR: 3.5–4.9), 3.2 (2.7–3.3), 3.0 (2.4–3.4) µg/ml. Our anesthesia protocol provides stable and reliable anesthesia in mechanically ventilated rats for several hours.
DOI of the first publication: 10.1538/expanim.21-0147
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-363615
ISSN: 1881-7122
Date of registration: 3-Jun-2022
Description of the related object: Supplementary material
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Anästhesiologie
Professorship: M - Prof. Dr. Thomas Volk
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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