Please use this identifier to cite or link to this item: doi:10.22028/D291-36340
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Title: Inhibition of erythropoietin-producing hepatoma receptor B4 (EphB4) signalling suppresses the vascularisation and growth of endometriotic lesions
Author(s): Rudzitis-Auth, Jeannette
Fuß, Sophia A.
Becker, Vivien
Menger, Michael D.
Laschke, Matthias W.
Language: English
Title: British Journal of Pharmacology
Volume: 177
Issue: 14
Pages: 3225-3239
Publisher/Platform: Wiley
Year of Publication: 2020
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background and Purpose:The development of endometriotic lesions is cruciallydependent on the formation of new blood vessels. In the present study, we analysedwhether this process is regulated by erythropoietin-producing hepatoma receptor B4(EphB4) signalling.Experimental Approach:We first assessed the anti-angiogenic action of the EphB4inhibitor NVP-BHG712 in different in vitro angiogenesis assays. Then, endometrioticlesions were surgically induced in the dorsal skinfold chamber and peritonealcavity of NVP-BHG712- or vehicle-treated BALB/c mice. This allowed to study theeffect of EphB4 inhibition on their vascularisation and growth by means of intravitalfluorescence microscopy, high-resolution ultrasound imaging, histology andimmunohistochemistry.Key Results:Non-cytotoxic doses of NVP-BHG712 suppressed the migration, tubeformation and sprouting activity of both human dermal microvascular endothelialcells (HDMEC) and mouse aortic rings. Accordingly, we also detected a lower bloodvessel density in NVP-BHG712-treated endometriotic lesions. This was associatedwith a reduced lesion growth due to a significantly lower number of proliferatingstromal cells when compared to vehicle-treated controls.Conclusions and Implications:Inhibition of EphB4 signalling suppresses thevascularisation and growth of endometriotic lesions. Hence, EphB4 represents apromising pharmacological target for the treatment of endometriosis.
DOI of the first publication: 10.1111/bph.15044
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-363405
ISSN: 1476-5381
Date of registration: 2-Jun-2022
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Michael D. Menger
Collections:Die Universitätsbibliographie

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