Please use this identifier to cite or link to this item: doi:10.22028/D291-35963
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Title: Enhancing the Stabilization Potential of Lyophilization for Extracellular Vesicles
Author(s): Trenkenschuh, Eduard
Richter, Maximilian
Heinrich, Eilien
Koch, Marcus
Fuhrmann, Gregor
Friess, Wolfgang
Language: English
Title: Advanced healthcare materials
Volume: 11
Issue: 5
Publisher/Platform: Wiley
Year of Publication: 2022
Publikation type: Journal Article
Abstract: Extracellular vesicles (EV) are an emerging technology as immune therapeutics and drug delivery vehicles. However, EVs are usually stored at -80 °C which limits potential clinical applicability. Freeze-drying of EVs striving for long-term stable formulations is therefore studied. The most appropriate formulation parameters are identified in freeze-thawing studies with two different EV types. After a freeze-drying feasibility study, four lyophilized EV formulations are tested for storage stability for up to 6 months. Freeze-thawing studies revealed improved colloidal EV stability in presence of sucrose or potassium phosphate buffer instead of sodium phosphate buffer or phosphate-buffered saline. Less aggregation and/or vesicle fusion occurred at neutral pH compared to slightly acidic or alkaline pH. EVs colloidal stability can be most effectively preserved by addition of low amounts of poloxamer 188. Polyvinyl pyrrolidone failed to preserve EVs upon freeze-drying. Particle size and concentration of EVs are retained over 6 months at 40 °C in lyophilizates containing 10 mm K- or Na-phosphate buffer, 0.02% poloxamer 188, and 5% sucrose. The biological activity of associated beta-glucuronidase is maintained for 1 month, but decreased after 6 months. Here optimized parameters for lyophilization of EVs that contribute to generate long-term stable EV formulations are presented.
DOI of the first publication: 10.1002/adhm.202100538
URL of the first publication:
Link to this record: hdl:20.500.11880/32786
ISSN: 2192-2659
Date of registration: 12-Apr-2022
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Jun.-Prof. Dr. Gregor Fuhrmann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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