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doi:10.22028/D291-35963
Titel: | Enhancing the Stabilization Potential of Lyophilization for Extracellular Vesicles |
VerfasserIn: | Trenkenschuh, Eduard Richter, Maximilian Heinrich, Eilien Koch, Marcus Fuhrmann, Gregor Friess, Wolfgang |
Sprache: | Englisch |
Titel: | Advanced healthcare materials |
Bandnummer: | 11 |
Heft: | 5 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2022 |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Extracellular vesicles (EV) are an emerging technology as immune therapeutics and drug delivery vehicles. However, EVs are usually stored at -80 °C which limits potential clinical applicability. Freeze-drying of EVs striving for long-term stable formulations is therefore studied. The most appropriate formulation parameters are identified in freeze-thawing studies with two different EV types. After a freeze-drying feasibility study, four lyophilized EV formulations are tested for storage stability for up to 6 months. Freeze-thawing studies revealed improved colloidal EV stability in presence of sucrose or potassium phosphate buffer instead of sodium phosphate buffer or phosphate-buffered saline. Less aggregation and/or vesicle fusion occurred at neutral pH compared to slightly acidic or alkaline pH. EVs colloidal stability can be most effectively preserved by addition of low amounts of poloxamer 188. Polyvinyl pyrrolidone failed to preserve EVs upon freeze-drying. Particle size and concentration of EVs are retained over 6 months at 40 °C in lyophilizates containing 10 mm K- or Na-phosphate buffer, 0.02% poloxamer 188, and 5% sucrose. The biological activity of associated beta-glucuronidase is maintained for 1 month, but decreased after 6 months. Here optimized parameters for lyophilization of EVs that contribute to generate long-term stable EV formulations are presented. |
DOI der Erstveröffentlichung: | 10.1002/adhm.202100538 |
URL der Erstveröffentlichung: | https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100538 |
Link zu diesem Datensatz: | hdl:20.500.11880/32786 http://dx.doi.org/10.22028/D291-35963 |
ISSN: | 2192-2659 2192-2640 |
Datum des Eintrags: | 12-Apr-2022 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Pharmazie |
Professur: | NT - Jun.-Prof. Dr. Gregor Fuhrmann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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