Please use this identifier to cite or link to this item: doi:10.22028/D291-35960
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Title: Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase
Author(s): Mehanny, Mina
Kroniger, Tobias
Koch, Marcus
Hoppstädter, Jessica
Becher, Dörte
Kiemer, Alexandra
Lehr, Claus-Michael
Fuhrmann, Gregor
Language: English
Title: Advanced healthcare materials
Volume: 11
Issue: 5
Publisher/Platform: Wiley
Year of Publication: 2022
Publikation type: Journal Article
Abstract: Streptococcus pneumoniae infections are a leading cause of death worldwide. Bacterial membrane vesicles (MVs) are promising vaccine candidates because of the antigenic components of their parent microorganisms. Pneumococcal MVs exhibit low toxicity towards several cell lines, but their clinical translation requires a high yield and strong immunogenic effects without compromising immune cell viability. MVs are isolated during either the stationary phase (24 h) or death phase (48 h), and their yields, immunogenicity and cytotoxicity in human primary macrophages and dendritic cells have been investigated. Death-phase vesicles showed higher yields than stationary-phase vesicles. Both vesicle types displayed acceptable compatibility with primary immune cells and several cell lines. Both vesicle types showed comparable uptake and enhanced release of the inflammatory cytokines, tumor necrosis factor and interleukin-6, from human primary immune cells. Proteomic analysis revealed similarities in vesicular immunogenic proteins such as pneumolysin, pneumococcal surface protein A, and IgA1 protease in both vesicle types, but stationary-phase MVs showed significantly lower autolysin levels than death-phase MVs. Although death-phase vesicles produced higher yields, they lacked superiority to stationary-phase vesicles as vaccine candidates owing to their similar antigenic protein cargo and comparable uptake into primary human immune cells.
DOI of the first publication: 10.1002/adhm.202101151
URL of the first publication: https://onlinelibrary.wiley.com/doi/10.1002/adhm.202101151
Link to this record: hdl:20.500.11880/32783
http://dx.doi.org/10.22028/D291-35960
ISSN: 2192-2659
2192-2640
Date of registration: 12-Apr-2022
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Jun.-Prof. Dr. Gregor Fuhrmann
NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Claus-Michael Lehr
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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