Please use this identifier to cite or link to this item: doi:10.22028/D291-35967
Title: In Vitro Erythropoiesis at Different pO2 Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
Author(s): Simionato, Greta
Rabe, Antonia
Gallego-Murillo, Joan Sebastián
van der Zwaan, Carmen
Hoogendijk, Arie Johan
van den Biggelaar, Maartje
Minetti, Giampaolo
Bogdanova, Anna
Mairbäurl, Heimo
Wagner, Christian
Kaestner, Lars
van den Akker, Emile
Language: English
Title: Cells
Volume: 11
Issue: 7
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: in vitro erythropoiesis
high altitude
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Hypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia and related EPO adjustments are not fully understood, which has contributed to the formulation of the theory of neocytolysis. We aimed to evaluate the role of oxygen exclusively on erythropoiesis, comparing in vitro erythroid differentiation performed at atmospheric oxygen, a lower oxygen concentration (three percent oxygen) and with cultures of erythroid precursors isolated from peripheral blood after a 19-day sojourn at high altitude (3450 m). Results highlight an accelerated erythroid maturation at low oxygen and more concave morphology of reticulocytes. No differences in deformability were observed in the formed reticulocytes in the tested conditions. Moreover, hematopoietic stem and progenitor cells isolated from blood affected by hypoxia at high altitude did not result in different erythroid development, suggesting no retention of a high-altitude signature but rather an immediate adaptation to oxygen concentration. This adaptation was observed during in vitro erythropoiesis at three percent oxygen by a significantly increased glycolytic metabolic profile. These hypoxia-induced effects on in vitro erythropoiesis fail to provide an intrinsic explanation of the concept of neocytolysis.
DOI of the first publication: 10.3390/cells11071082
Link to this record: urn:nbn:de:bsz:291--ds-359670
ISSN: 2073-4409
Date of registration: 12-Apr-2022
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Christian Wagner
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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