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Titel: An Outer Membrane Vesicle-Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability
VerfasserIn: Richter, Robert
Kamal, Mohamed A. M.
Koch, Marcus
Niebuur, Bart-Jan
Huber, Anna-Lena
Goes Conceicao, Adriely
Volz, Carsten
Vergalli, Julia
Kraus, Tobias
Müller, Rolf
Schneider-Daum, Nicole
Fuhrmann, Gregor
Pagès, Jean-Marie
Lehr, Claus-Michael
Sprache: Englisch
Titel: Advanced healthcare materials
Bandnummer: 11
Heft: 5
Verlag/Plattform: Wiley
Erscheinungsjahr: 2021
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: When searching for new antibiotics against Gram-negative bacterial infections, a better understanding of the permeability across the cell envelope and tools to discriminate high from low bacterial bioavailability compounds are urgently needed. Inspired by the phospholipid vesicle-based permeation assay (PVPA), which is designed to predict non-facilitated permeation across phospholipid membranes, outer membrane vesicles (OMVs) of Escherichia coli either enriched or deficient of porins are employed to coat filter supports for predicting drug uptake across the complex cell envelope. OMVs and the obtained in vitro model are structurally and functionally characterized using cryo-TEM, SEM, CLSM, SAXS, and light scattering techniques. In vitro permeability, obtained from the membrane model for a set of nine antibiotics, correlates with reported in bacterio accumulation data and allows to discriminate high from low accumulating antibiotics. In contrast, the correlation of the same data set generated by liposome-based comparator membranes is poor. This better correlation of the OMV-derived membranes points to the importance of hydrophilic membrane components, such as lipopolysaccharides and porins, since those features are lacking in liposomal comparator membranes. This approach can offer in the future a high throughput screening tool with high predictive capacity or can help to identify compound- and bacteria-specific passive uptake pathways.
DOI der Erstveröffentlichung: 10.1002/adhm.202101180
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/10.1002/adhm.202101180
Link zu diesem Datensatz: hdl:20.500.11880/32724
http://dx.doi.org/10.22028/D291-35067
ISSN: 2192-2659
2192-2640
Datum des Eintrags: 5-Apr-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
NT - Pharmazie
Professur: NT - Prof. Dr. Tobias Kraus
NT - Prof. Dr. Claus-Michael Lehr
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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